COX IV Antikörper (AA 154-166)

Produktnummer ABIN461950

Dieser Ziege Polyklonal Antikörper detektiert spezifisch COX IV in WB, IHC, ELISA und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human und Affe.

Kurzübersicht für COX IV Antikörper (AA 154-166) (ABIN461950)

Target: COX IV (COX4I1) (Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))

Reaktivität: Human, Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser COX IV Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-COX IV Antikörper

Bindungsspezifität: AA 154-166

Spezifität: Human COX4I1. This antibody is expected to recognize COX4I1 (GeneID:1327, MW: 19.6  kDa) & COX4I2 (GeneID:84701, MW:19.6  kDa).

Homologie: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%) Panda, Dog, Bat, Xenopus (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-QGLASKWDYEKNE from the C-terminus of human COX4I1 (NP_001852.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%), Panda, Dog, Bat, Xenopus (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:4000), WB (1:4000, 0.01 - 0.03 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu COX IV

Target: COX IV (COX4I1) (Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))

Andere Bezeichnung: COXIV / COX4

Hintergrund: Name/Gene ID: COX4I1

Synonyms: COX4I1, COX4-1, COX4, COX IV-1, COXIV

Gen-ID: 1327

NCBI Accession: NP_001852

UniProt: P13073

Pathways: Proton Transport