beta Amyloid Antikörper

Produktnummer ABIN459388

Dieser Maus Monoklonal Antikörper detektiert spezifisch beta Amyloid in ELISA, IHC und IF. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für beta Amyloid Antikörper (ABIN459388)

Target: beta Amyloid (Abeta) (Amyloid beta (Abeta))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser beta Amyloid Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Klon: OMAB

Produktdetails anti-beta Amyloid Antikörper

Verwendungszweck: Anti-Amyloid beta oligomer-specific monoclonal antibody (OMAB)

Kreuzreaktivität (Details): Human Abeta oligomers only
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Homologie: Rat

Aufreinigung: Affinity purified

Immunogen: Partly aggregated

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: Coating antibody at 2 μg/mL (ELISA), 1: 1000 (IF), 1 : 500 (IHC)

Kommentare:

OMAB antibody is a versatile tool within research of Alzheimer's disease. A sandwhich ELISA illustrates its potential regarding its high selectivity towards Aβ oligomers.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 μL of sterile water

Buffer: Affinity purified in PBS pH 7.4.

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: 4 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at 4°C. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Details zu beta Amyloid

Target: beta Amyloid (Abeta) (Amyloid beta (Abeta))

Andere Bezeichnung: Amyloid beta oligomer-specific

Hintergrund:

Background: Soluble oligomeric assemblies of the Amyloid-β peptide are today anticipated to be the direct cause regarding the Alzheimer pathology. As a consequence, oligomeric Aβ-assemblies constitute a very interesting therapeutic target. Identification of Aβ-oligomers is however, technically challenging due to there labile nature and low abundance. Abeta oligomer-specific OMAB antibody is based on the IgM isotype and represents a new concept of Aβ-oligomer binders using a combination of high avidity and very low monovalent affinity. This combination creates a selectivity of the antibody towards the oligomeric fraction and minimizes reactivity towards monomeric species.

Additional information: OMAB antibody has been purified by by ion-exchange chromatography and is supplied in PBS without any additives as carrier proteins or sodium azide.Binding of OMAB antibody and Abeta oligomers at RT takes about 15 min.Fibrils are inaccessible for OMAB antibodies therefore if a discrimination between fibrils and oligomers is to be achieved, dot blot can be used. Start with antigen concentration of 500 ng/dot followed by 2X dilution steps. Blocking: non-fat milk and washes with 0.3 % Tween 20 in TBS pH 7.4.

Molekulargewicht: 4.5 kDa

Pathways: Inflammasome