GAPDH Antikörper (Biotin)

Produktnummer ABIN459124

Dieser Schaf Polyklonal Antikörper detektiert spezifisch GAPDH in WB, ELISA, ICC, IHC (p) und DB. Es zeigt Reaktivität gegenüber Proben von Kaninchen.

Kurzübersicht für GAPDH Antikörper (Biotin) (ABIN459124)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reaktivität: Kaninchen

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser GAPDH Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Dot Blot (DB)

Produktdetails anti-GAPDH Antikörper (Biotin)

Verwendungszweck: Sheep anti Rabbit Glyceraldehyde-3-phosphate dehydrogenase, conjugated with Biotin

Spezifität: Biotin-conjugated IgG fraction of polyclonal Sheep antiSerum to glyceraldehyde-3- phosphate dehydrogenase from Rabbit muscle

Kreuzreaktivität (Details): Cross-reactivities against enzymes of other sources may occur but have not been determined.

Produktmerkmale: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).

Aufreinigung: Purified IgG fraction from antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: Glyceraldehyde-3-phosphate dehydrogenase isolated and purified from rabbit muscle. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is reconstituted by adding 1.0 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: Biotin-coupled hyperimmune sheep IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Details zu GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Andere Bezeichnung: Glyceraldehyde-3-phosphate Dehydrogenase

UniProt: P46406