PFKM Antikörper (Biotin)

Produktnummer ABIN459123

Dieses Anti-PFKM-Antikörper ist ein Schaf Polyklonal-Antikörper zur Detektion von PFKM in WB, ELISA und IF. Geeignet für Kaninchen.

Kurzübersicht für PFKM Antikörper (Biotin) (ABIN459123)

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Reaktivität: Kaninchen

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser PFKM Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Produktdetails anti-PFKM Antikörper (Biotin)

Spezifität: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Produktmerkmale: Biotin-conjugated IgG fraction of polyclonal sheep antiserum to fructose-6-phosphate kinase from rabbit muscle

Aufreinigung: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: Fructose-6-phosphate kinase isolated and purified from rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: IgG protein concentration 10 mg/ml. Biotin/ IgG protein molar ratio (B/P) approximately 6.5. No foreign proteins added.

Buffer: Biotin-coupled hyperimmune sheep IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Details zu PFKM

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Andere Bezeichnung: Fructose-6-phosphate Kinase

Hintergrund: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.

Pathways: Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Warburg Effekt