IgA Secretory Component (Free & Bound) Antikörper

Produktnummer ABIN458766

Der Ziege Polyklonal anti-IgA Secretory Component Antikörper (ABIN458766) detektiert spezifisch IgA Secretory Component in ELISA, WB, IFA und DB. Dieser Antikörper reagiert spezifisch mit Proben aus Affe.

Kurzübersicht für IgA Secretory Component (Free & Bound) Antikörper (ABIN458766)

Target: IgA Secretory Component

Reaktivität: Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: ELISA, Western Blotting (WB), Indirect Immunofluorescence Assay (IFA), Dot Blot (DB)

Produktdetails

Bindungsspezifität: Free & Bound

Verwendungszweck: Goat anti Monkey secretory component (free and bound)

Spezifität: Purified IgG fraction of polyclonal Goat antiSerum to rhesus Monkey secretory component, free and bound.

Kreuzreaktivität (Details): Inter-species cross-reactivity is a normal feature of antibodies to Serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent of the concentRation of the reactants and he sensitivity of the method. This product may also react with free and bound secretory component in many old-world Monkeys, including Macaca Sylvanus, Cercopithecus, Cynomolgus, Baboon, and also with the secretory component of Chimpanzees. In double radial immunodiffusion a reaction has been observed with free Human secretory component.

Produktmerkmale: Tested in immunoelectrophoresis and double radial immunodiffusion against a panel of appropriate secretions and purified immunoglobulin isotypes. The antiserum reacts with both bound secretory component (secretory IgA) and with free secretory component. In immunoelectrophoresis against normal monkey milk, using a high electroendosmosis agar plate, free secretory component ism precipitated in the alpha-2 region. This antiserum does not react with other molecular forms of IgA, or with any other secretory or plasma protein and double radial immunodiffusion. As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.

Aufreinigung: Purified IgG fraction from antiserum

Immunogen: Secretory component can be present in monkey secretions bound to secretory IgA (sIgA) and in free form. Free monkey secretory component isolated from pooled milk is used for immunization. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Antigendetails

Target: IgA Secretory Component

Andere Bezeichnung: Secretory Immunoglobulin A (SIgA)