IgA Secretory Component (Free & Bound) Antikörper (Biotin)

Produktnummer ABIN458285

Der Ziege Polyklonal anti-IgA Secretory Component Antikörper wird verwendet zum Nachweis von IgA Secretory Component in Proben von Affe. Er wurde validiert für ELISA, WB, IHC (p), ICC und DB.

Kurzübersicht für IgA Secretory Component (Free & Bound) Antikörper (Biotin) (ABIN458285)

Target: IgA Secretory Component

Reaktivität: Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Biotin

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Dot Blot (DB)

Produktdetails

Bindungsspezifität: Free & Bound

Verwendungszweck: Goat anti Monkey secretory component (free and bound), conjugated with Biotin

Spezifität: Biotin-conjugated IgG fraction of polyclonal Goat antiSerum to Monkey secretory component, free and bound

Kreuzreaktivität (Details): Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Precipitation reactions have been observed with free and bound secretory component in Serum of other old-world Monkeys, including Cercopithecus, Cynomolgus and Baboon. The conjugate may also react with other species as has been observed for Chimpanzee.

Produktmerkmale: Tested in immunoelectrophoresis, double radial immunodiffusion and ELISA against a panel of appropriate secretions and purified Ig isotypes. The conjugate reacts with both bound secretory component (secretory IgA) and with the free SC present in monkey secretions. In immunoelectrophoresis against monkey milk, using a high electroendosmosis agar plate, free SC is precipitated in the alph-2 region. The immunoconjugate does not react with other molecular forms of IgA, or with any other secretory or plasma protein. In immunocytochemical and immunohistochemical staining for the detection of free SC and secretory IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. In non-isotopic assay methodology (e.g. ELISA) to identify and measure SC or secretory IgA in monkey body fluids. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Aufreinigung: Purified

Immunogen: Secretory component is present in monkey secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65,000). The dimmer IgA is transported into secretions by its binding to the SC on the epithelial cells. SC also has an affinity for polymeric IgM. Purified free monkey secretory component isolated from pooled milk is used for immunization. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: Biotin-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Antigendetails

Target: IgA Secretory Component

Andere Bezeichnung: Secretory Immunoglobulin A (SIgA)