Albumin Antikörper

Produktnummer ABIN458755

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Albumin in WB, ELISA, DB und IFA. Es zeigt Reaktivität gegenüber Proben von Ratte.

Kurzübersicht für Albumin Antikörper (ABIN458755)

Target: Albumin (ALB)

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Albumin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Dot Blot (DB), Indirect Immunofluorescence Assay (IFA)

Produktdetails anti-Albumin Antikörper

Verwendungszweck: Rabbit anti Rat albumin

Spezifität: Purified IgG fraction of polyclonal Rabbit antiSerum to Rat albumin.

Kreuzreaktivität: Maus, Schwein

Keine Kreuzreaktivität: Huhn, Rind (Kuh), Hund, Ziege, Kaninchen, Schaf

Kreuzreaktivität (Details): Inter-species cross-reactivity is a normal feature of antibodies to mammalian Serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. This antiSerum fraction has been tested for cross-reactivity by double radial immunodiffusion anti several species sera with the following results: Bovine - Chicken - Dog - Goat - Guinea Pig ++ Horse ± Human ± Monkey ± Mouse ++ Rabbit - Sheep - Swine ±

Produktmerkmale: The defined antibody specificity is directed to albumin as tested against rat sera. In immunoelectrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against rat serum and the purified albumin The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates, in radioimmunoassay, for the production of immunoconjugates with a selected marker, to prepare immunoaffinity adsorbents by coupling to an artificial carrier, in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent, in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.

Aufreinigung: Purified IgG fraction from antiserum

Immunogen: Highly purified albumin isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Details zu Albumin

Target: Albumin (ALB)

Andere Bezeichnung: Albumin

Pathways: Lipid Metabolism