Albumin Antikörper (HRP)

Produktnummer ABIN458654

Der Ziege Polyklonal anti-Albumin Antikörper wird verwendet zum Nachweis von Albumin in Proben von Ratte. Er wurde validiert für WB, ELISA, ICC, IHC (p) und DB.

Kurzübersicht für Albumin Antikörper (HRP) (ABIN458654)

Target: Albumin (ALB)

Reaktivität: Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Albumin Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Dot Blot (DB)

Produktdetails anti-Albumin Antikörper (HRP)

Verwendungszweck: Goat anti Rat albumin, conjugated with Horseradish peroxidase

Spezifität: Horseradish peroxidase-conjugated IgG fraction of polyclonal Goat antiSerum to Rat albumin.

Kreuzreaktivität: Hund, Pferd, Human, Affe, Maus, Schwein

Keine Kreuzreaktivität: Huhn, Rind (Kuh), Ziege, Schaf

Kreuzreaktivität (Details): Inter-species cross-reactivity is a normal feature of antibodies to mammalian Serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. This antiSerum fraction has been tested for cross-reactivity by double radial immunodiffusion anti several species sera with the following results: Bovine - Goat - Human + Rabbit ± Chicken - Guinea Pig ++ Mouse ++ Sheep - Dog + Horse + Monkey + Swine + A negative reaction in double radial immunodiffusion does not exclude a weak positive reaction in more sensitive techniques.

Produktmerkmale: Tested in immunoelectrophoresis and double radial immunodiffusion against pooled normal rat serum and purified rat albumin. One characteristic precipitin line is obtained against pooled normal rat serum using different antigen/antibody concentration ratio's. Precipitin lines against normal rat serum and purified rat albumin give a reaction of full identity. In enzyme-immunocytochemical and immunohistochemical staining for the detection of albumin, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure albumin in rat serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Aufreinigung: Purified IgG fraction from antiserum

Immunogen: Purified albumin isolated from pooled rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Konservierungsmittel: Without preservative

Details zu Albumin

Target: Albumin (ALB)

Andere Bezeichnung: Albumin

UniProt: P02770

Pathways: Lipid Metabolism