Complement C3c Antikörper

Produktnummer ABIN458242

Dieser Schwein Polyklonal Antikörper detektiert spezifisch Complement C3c in ELISA, ICC und IF. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Complement C3c Antikörper (ABIN458242)

Target: Complement C3c (C3C) (Complement Component C3c (C3C))

Reaktivität: Human

Wirt: Schwein

Klonalität: Polyklonal

Konjugat: Dieser Complement C3c Antikörper ist unkonjugiert

Applikation: ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Produktdetails anti-Complement C3c Antikörper

Spezifität: The antiserum does not cross-react with any other component of human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. of this antiserum has not been tested in detail.

Produktmerkmale: Purified IgG fraction of polyclonal swine antiserum to C3c fragment of human complement factor C3

Aufreinigung: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. The IgG (7S) fraction is isolated and purified from the antiserum and contains the bulk of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis and double radial immunodiffusion.

Immunogen: C3c is the major fragment resulting from C3 cleavage by C3 convertase and factor I. It is composed of an intact beta chain bound to two fragments of the alpha chain. C3c is isolated and purified from pooled normal human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: As unlabelled primary or secondary antibody for the detection of C3c at the cellular and subcellular level by immunofluorescence and immunoenzyme assay methods, for the production of immunoconjugates with a selected marker, to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier, as catching or detecting antibody in non-isotopic methodology and solid phase immunochemistry. Determinations of individual complement components can be useful in defining the exact location of a defect. When applied in any immunocytochemical or histochemical staining procedure or solid phase coupling technique, the optimum working dilution should be established by titration before being used. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250, in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: IgG concentration 10 mg/ml. No foreign proteins added. Antibody titre: Precipitin titre not lees than 1:16 when tested against normal human serum in agar block immunodiffusion titration.

Buffer: Purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2)

Konservierungsmittel: Without preservative

Lagerung: 4 °C

Informationen zur Lagerung: The IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile di stilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, a nd preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Details zu Complement C3c

Target: Complement C3c (C3C) (Complement Component C3c (C3C))

Andere Bezeichnung: C3c Fragment of Complement Factor C3

Hintergrund: In immunoelectrophoresis against fresh human serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c cab also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other proteins component of human serum or plasma