Complement C2 Antikörper

Produktnummer ABIN458139

Dieses Anti-Complement C2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Complement C2 in IEP. Geeignet für Human.

Kurzübersicht für Complement C2 Antikörper (ABIN458139)

Target: Complement C2

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Complement C2 Antikörper ist unkonjugiert

Applikation: Immunoelectrophoresis (IEP)

Produktdetails anti-Complement C2 Antikörper

Spezifität: The antiserum does not cross-react with any other human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.

Produktmerkmale: Precipitating polyclonal rabbit antiserum to human C2 component of complement

Aufreinigung: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: The C2 component of human complement is a single-chain polypeptide with a molecular weight of 110,000. It is present in plasma in an average concentration of 25 µ g/ml. The activated form of C2 combines with the activated C4 to form a complex with C3-convertase activity in the classical pathway of complement activation. C2 is isolated as a homogenous protein for use in the antiserum production. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: In precipitating techniques as immunoelectrophoresis and single and double radial immuno-diffusion (Mancini, Ouchterlony) to identify the presence of C2 or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: The IgG concentration is 4 mg/ml in PBS-EDTA. No foreign proteins added. Antibody titre: Precipitin titre not less than 1:32 when tested against appropriate concentrations of purified C2 protein in agar-block immunodiffusion titration.

Buffer: Delipidated, heat inactivated, lyophilized, stable IgG fraction.

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.

Details zu Complement C2

Target: Complement C2

Andere Bezeichnung: C2 Component of Complement

Hintergrund: The reactivity of the antiserum is restricted to native and activated C2. In immunoelectro-phoresis and radial immunodiffusion, using various antiserum concentrations against human plasma a single precipitin line is obtained and shows a reaction of full identity with the purified immunogen