Complement C2 Antikörper

Produktnummer ABIN337358

Produktdetails anti-Complement C2 Antikörper

Target: Complement C2

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Complement C2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Recognizes C2 from human plasma/serum. Epitope specificity differs from that of LS-C22277 and LS-C22278 as determined by inhibition ELISA. Reacts strongly with C2. Strong reaction is seen with human serum/plasma and no reaction is seen with serum from patients deficient in C2, when tested in sandwich ELISA with a polyclonal antibody against C2. In ELISA, reaction is also seen with C2 directly coated onto the microtiter well.

Aufreinigung: Protein A/G purified

Immunogen: Complement component C2 isolated from human plasma.

Type of Immunogen: Purified protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:2000), IHC, IHC-P (10 μg/mL), WB (1:125)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: After SDS-PAGE, this antibody reacts with C2 and a subfraction of C2 believed to be C2b, both under reducing and non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu Complement C2

Target: Complement C2

Abstract: Complement C2 Produkte

Hintergrund: Name/Gene ID: C2
Subfamily: Serine S1
Family: Protease

Synonyms: C2, C2b, C3/C5 convertase, Complement component C2, Complement C2, Complement component 2, CO2

Gen-ID: 717

UniProt: P06681