Factor XI Antikörper

Produktnummer ABIN458046

Produktdetails anti-Factor XI Antikörper

Target: Factor XI (F11) (Coagulation Factor XI (F11))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Factor XI Antikörper ist unkonjugiert

Applikation: Immunoelectrophoresis (IEP), Ouchterlony Double Diffusion (ODD)

Spezifität: The antiserum does not cross-react with any other human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.

Produktmerkmale: Precipitating polyclonal goat antiserum to human coagulation Factor XI

Aufreinigung: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: Factor XI is a glycoprotein (MW 160,000) composed of two identical polypeptide chains. In electrophoresis it migrates in the gamma region. It is stable on storage, heat stable at 56ºC for 30 minutes and not adsorbed by gels. Factor XI also occurs in platelets (MW 52,000). Factor XI is converted to an active enzyme by FXIIa but can be activated by other proteases such as trypsin. FXIa activates FIX, but can also activate FXII and plasminogen. Platelet membrane FXI can substitute for plasma FXI in coagulation. Plasma FXI is used for immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This antiserum is primarily intended for the measurement of Factor XI in human plasma using the EID-method (Laurell). FXI is one of the four proteins known to be involved in normal contact activation reactions, activating FXII to FXIIa, which is further capable of triggering the kinin-forming pathway and the fibrinolytic system. FXI is present in normal human plasma both in free form and as a reversible complex with HMWK. FXI competes with prekallikrein (PKK) to form such complexes. Since FXI is a gammaglobulin with cathodic mobility in electrophoresis, up to 140 µ g/ml purified HMWK must be added to the plasma to bind all free FXI into negatively charged complexes which will migrate to the anode. The total FXI content can then be measured. The average amount of FXI in an individual plasma was found to be 4.5 µ g/ml with a range of 3 to 6 µ g/ml. The sensitivity of the EIID method is about 0.2 µ g/ml. A congenital deficiency of FXI in plasma can give rise to a serious bleeding disorder. In most cases the plasma level of FXI antigen is also depressed, exceptionally the FXI antigen is normal ( a defective molecule) An acquired reduction in both clotting activity and FXI antigen level has been described in disseminated intravascular coagulopathy, in hepatic cirrhosis and in some patients with lupus erythomatosus.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added. Antibody titre: Precipitin titre not less than 1:32 as measured against appropriate antigens in precipitin-block titratio

Buffer: Delipidated, heat inactivated, lyophilized, stable whole serum.

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same da y.

Details zu Factor XI

Target: Factor XI (F11) (Coagulation Factor XI (F11))

Andere Bezeichnung: Coagulation Factor XI (F11 Produkte)

Synonyme: FXI antikoerper, 1600027G01Rik antikoerper, AI503996 antikoerper, Cf11 antikoerper, LRRGT00086 antikoerper, coagulation factor XI antikoerper, F11 antikoerper, CpipJ_CPIJ000593 antikoerper, CpipJ_CPIJ001060 antikoerper, CpipJ_CPIJ002752 antikoerper, CpipJ_CPIJ003631 antikoerper, CpipJ_CPIJ004041 antikoerper, CpipJ_CPIJ004093 antikoerper

Hintergrund: The defined antibody reactivity is restricted to FXI, FXIa and complexes of FXI with HMWK or other proteins. In immunoelectrophoresis, bi-dimensional electrophoresis and radial immunodiffusion (Ouchterlony) against normal plasma, a single precipitin line is obtained which shows a reaction of identity with precipitated purified Factor XI. No reaction is obtained with FXI-depleted plasma