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Albumin Antikörper (Biotin)

ALB Reaktivität: Human ELISA, IHC, ICC Wirt: Ziege Polyclonal Biotin
Produktnummer ABIN457981
  • Target Alle Albumin (ALB) Antikörper anzeigen
    Albumin (ALB)
    Reaktivität
    • 164
    • 96
    • 95
    • 73
    • 39
    • 34
    • 33
    • 32
    • 28
    • 25
    • 22
    • 21
    • 21
    • 12
    • 9
    • 7
    • 7
    • 5
    • 2
    • 2
    • 2
    • 1
    Human
    Wirt
    • 339
    • 63
    • 62
    • 29
    • 11
    • 7
    • 2
    Ziege
    Klonalität
    • 443
    • 66
    • 1
    Polyklonal
    Konjugat
    • 209
    • 103
    • 78
    • 56
    • 8
    • 8
    • 8
    • 7
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Dieser Albumin Antikörper ist konjugiert mit Biotin
    Applikation
    • 288
    • 268
    • 185
    • 103
    • 102
    • 55
    • 41
    • 40
    • 35
    • 34
    • 32
    • 31
    • 31
    • 27
    • 19
    • 19
    • 18
    • 16
    • 16
    • 11
    • 10
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)
    Spezifität
    Inter-species cross-reactivity is a normal feature of antibodies to mammalian serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum fraction has not been tested in detail.
    Produktmerkmale
    Biotin-conjugated IgG fraction of polyclonal goat antiserum to human albumin
    Aufreinigung
    Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.
    Immunogen
    Albumin is a stable small polypeptide with a strong antigenicity. Its molecular weight is about 69,000. It has a high mobility in electrophoresis, shows macro-heterogeneity especially under pathological con-ditions and it can bind a large number of physiological and non-physiological molecules. Albumin is isolated from human serum by sequential precipitation and purified by ion exchange chromatography and affinity chromatography. Freund’s complete adjuvant is used in the first step of the immunization procedure.
    Isotyp
    IgG
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  • Applikationshinweise
    In immunocytochemical and immunohistochemical use for the detection of albumin at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, in non-isotopic assay methodology (e.g. ELISA) to measure albumin in human serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1: 500, in ELISA and comparable non-precipitating antibody-binding assays between 1:5,000 and 1:50,000.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Konzentration
    IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 5.3. No foreign proteins added.
    Buffer
    Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
    Konservierungsmittel
    Without preservative
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    The lyophilized immunoconjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile di stilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
  • Target
    Albumin (ALB)
    Andere Bezeichnung
    Albumin (ALB Produkte)
    Synonyme
    PRO0883 antikoerper, PRO0903 antikoerper, PRO1341 antikoerper, ALB antikoerper, CSA antikoerper, Alb-1 antikoerper, Alb1 antikoerper, Albza antikoerper, LOC100136344 antikoerper, alb-a antikoerper, alb-b antikoerper, albumin antikoerper, serum albumin 1 antikoerper, albumin S homeolog antikoerper, ALB antikoerper, Alb antikoerper, LOC100136344 antikoerper, alb.S antikoerper
    Hintergrund
    The defined antibody specificity is directed to albumin as tested against human sera. In immuno- electrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against human serum and the purified albumin.
    Pathways
    Lipid Metabolism
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