Complement C3c Antikörper

Produktnummer ABIN457647

Der Ziege Polyklonal anti-Complement C3c Antikörper (ABIN457647) detektiert spezifisch Complement C3c in IEP. Dieser Antikörper reagiert spezifisch mit Proben aus Hund.

Kurzübersicht für Complement C3c Antikörper (ABIN457647)

Target: Complement C3c (C3C) (Complement Component C3c (C3C))

Reaktivität: Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Complement C3c Antikörper ist unkonjugiert

Applikation: Immunoelectrophoresis (IEP)

Produktdetails anti-Complement C3c Antikörper

Spezifität: Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.

Produktmerkmale: Precipitating polyclonal goat antiserum to C3c subunit of dog complement C3

Aufreinigung: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other dog serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: C3c is the major fragment resulting from the C3 cleavage by C3 convertase and factor i. It is composed of an intact beta chain bound to two fragments of the alpha chain. The protein is isolated and purified from pooled normal dog serum by precipitation techniques, followed by chromatographical methods. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: In precipitating techniques as immunoelectrophoresis and single and double radial immuno-diffusion (Mancini, Ouchterlony) to identify the presence of complement C3c or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included. Determinations of individual complement components can be very useful in defining the exact location of a defect.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled goat serum. No foreign proteins added. Antibody titre: Precipitin titre not less than 1:128 when tested against normal dog plasma in agar-block immunodiffusion

Buffer: Delipidated, heat inactivated, lyophilized, stable whole serum.

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.

Details zu Complement C3c

Target: Complement C3c (C3C) (Complement Component C3c (C3C))

Andere Bezeichnung: C3c Subunit of Complement C3

Hintergrund: In immunoelectrophoresis against fresh dog serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c can also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other proteins component of dog serum or plasma