Albumin Antikörper (FITC)

Produktnummer ABIN457584

Der FITC-konjugierte Kaninchen Polyklonal anti-Albumin Antikörper (ABIN457584) detektiert spezifisch Albumin in ELISA, ICC, IHC (fro) und IFA. Dieser Antikörper reagiert spezifisch mit Proben aus Rind (Kuh).

Kurzübersicht für Albumin Antikörper (FITC) (ABIN457584)

Target: Albumin (ALB)

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Albumin Antikörper ist konjugiert mit FITC

Applikation: ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Indirect Immunofluorescence Assay (IFA)

Produktdetails anti-Albumin Antikörper (FITC)

Verwendungszweck: Rabbit anti Bovine albumin, conjugated with FITC

Spezifität: Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Rabbit antiSerum to Bovine albumin.

Kreuzreaktivität: Hund, Ziege, Hamster, Pferd, Human, Affe, Maus, Schwein

Keine Kreuzreaktivität: Cat, Huhn, Ente, Kaninchen, Schaf

Kreuzreaktivität (Details): Inter-species cross-reactivity is a normal feature of antibodies to mammalian Serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of crossreactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. This antiSerum has been tested in double radial immunodiffusion with the following results: Cat - Chicken - Dog + Duck - Goat ++ Guinea Pig ++ Hamster + Horse + Human + Monkey + Mouse ++ pigeon - Rabbit - Rat ± Sheep - Swine + A negative cross-reaction in double radial immunodiffusion does not exclude some reaction in more sensitive techniques.

Produktmerkmale: The defined antibody specificity is directed to albumin as tested against bovine sera. In immunoelectrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against bovine serum and the purified albumin. As reagent for the direct detection of albumin in bovine cells, tissues and body fluids in immunofluorescence techniques. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Aufreinigung: Purified IgG fraction from antiserum

Immunogen: Albumin is a stable small polypeptide with a strong antigenicity. Its molecular weight is about 69,000. It has a high mobility in electrophoresis, shows macro-heterogeneity especially under pathological conditions and it can bind a large number of physiological and non-physiological molecules. Albumin is isolated from bovine serum by sequential precipitation and purified by ion exchange chromatography and affinity chromatography. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Details zu Albumin

Target: Albumin (ALB)

Andere Bezeichnung: Albumin

Pathways: Lipid Metabolism