CD86 Antikörper

Produktnummer ABIN457421

Produktdetails anti-CD86 Antikörper

Target: CD86

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD86 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Spezifität: The rat monoclonal antibody GL-1 reacts with an extracellular epitope of CD86 (B7-2), a 70-80 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.

Kreuzreaktivität (Details): Mouse

Aufreinigung: Purified by protein-G affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: LPS-activated CBA/Cs mouse splenic B cells

Klon: GL-1

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Recommended dilution: 2 μg/mL, positive control: murine splenocytes.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-CD86 Antikörper (ABIN457421)

Nolan, Kobayashi, Naveed, Kelly, Hoshino, Hoshino, Karulf, Rom, Weiden, Gold: "Differential role for CD80 and CD86 in the regulation of the innate immune response in murine polymicrobial sepsis." in: PLoS ONE, Vol. 4, Issue 8, pp. e6600, (2009) (PubMed).

Edgtton, Kausman, Li, OSullivan, Lo, Hutchinson, Yagita, Holdsworth, Kitching: "Intrarenal antigens activate CD4+ cells via co-stimulatory signals from dendritic cells." in: Journal of the American Society of Nephrology : JASN, Vol. 19, Issue 3, pp. 515-26, (2008) (PubMed).

Nolan, Weiden, Kelly, Hoshino, Hoshino, Mehta, Gold: "CD40 and CD80/86 act synergistically to regulate inflammation and mortality in polymicrobial sepsis." in: American journal of respiratory and critical care medicine, Vol. 177, Issue 3, pp. 301-8, (2008) (PubMed).

Steptoe, Ritchie, Jones, Harrison: "Autoimmune diabetes is suppressed by transfer of proinsulin-encoding Gr-1+ myeloid progenitor cells that differentiate in vivo into resting dendritic cells." in: Diabetes, Vol. 54, Issue 2, pp. 434-42, (2005) (PubMed).

Brasel, De Smedt, Smith, Maliszewski: "Generation of murine dendritic cells from flt3-ligand-supplemented bone marrow cultures." in: Blood, Vol. 96, Issue 9, pp. 3029-39, (2000) (PubMed).

Benschop, Melamed, Nemazee, Cambier: "Distinct signal thresholds for the unique antigen receptor-linked gene expression programs in mature and immature B cells." in: The Journal of experimental medicine, Vol. 190, Issue 6, pp. 749-56, (1999) (PubMed).

Hathcock, Laszlo, Dickler, Bradshaw, Linsley, Hodes: "Identification of an alternative CTLA-4 ligand costimulatory for T cell activation." in: Science (New York, N.Y.), Vol. 262, Issue 5135, pp. 905-7, (1993) (PubMed).

Details zu CD86

Target: CD86

Andere Bezeichnung: CD86 (CD86 Produkte)

Synonyme: B7-2 antikoerper, B7.2 antikoerper, B70 antikoerper, CD28LG2 antikoerper, LAB72 antikoerper, B7 antikoerper, CLS1 antikoerper, Cd28l2 antikoerper, ETC-1 antikoerper, Ly-58 antikoerper, Ly58 antikoerper, MB7 antikoerper, MB7-2 antikoerper, TS/A-2 antikoerper, CD86 molecule antikoerper, CD86 antigen antikoerper, integrin subunit alpha 2b antikoerper, CD86 antikoerper, Cd86 antikoerper, Itga2b antikoerper

Hintergrund: CD86 Molecule,CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 Molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet, there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.,B7-2, FUN-1, Ly58

Gen-ID: 12524

UniProt: P42082

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation