CD3 epsilon Antikörper (Activation Site) (PE)

Produktnummer ABIN457363

Produktdetails anti-CD3 epsilon Antikörper (PE)

Target: CD3 epsilon (CD3E)

Bindungsspezifität: Activation Site

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD3 epsilon Antikörper ist konjugiert mit PE

Applikation: Intracellular Flow Cytometry (ICFC)

Verwendungszweck: Anti-CD3 epsilon (activation epitope) PE

Spezifität: The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.

Kreuzreaktivität (Details): Human, Mouse

Aufreinigung: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Purified human CD3 proteins isolated from thymus

Klon: APA1-1

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.
Avoid prolonged exposure to light.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Referenzen für anti-CD3 epsilon Antikörper (PE) (ABIN457363)

Tailor, Tsai, Shameli, Serra, Wang, Robbins, Nagata, Szymczak-Workman, Vignali, Santamaria: "The proline-rich sequence of CD3epsilon as an amplifier of low-avidity TCR signaling." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 1, pp. 243-55, (2008) (PubMed).

Wong, Dutly, Sacher, Lee, Hwang, Liu, Keshavjee, Hu, Waddell: "Targeted cell replacement with bone marrow cells for airway epithelial regeneration." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 293, Issue 3, pp. L740-52, (2007) (PubMed).

Rieux-Laucat, Hivroz, Lim, Mateo, Pellier, Selz, Fischer, Le Deist: "Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency." in: The New England journal of medicine, Vol. 354, Issue 18, pp. 1913-21, (2006) (PubMed).

Gil, Schrum, Alarcón, Palmer: "T cell receptor engagement by peptide-MHC ligands induces a conformational change in the CD3 complex of thymocytes." in: The Journal of experimental medicine, Vol. 201, Issue 4, pp. 517-22, (2005) (PubMed).

Torres, Alcover, Zapata, Arnaud, Pacheco, Martín-Fernández, Villasevil, Sanal, Regueiro: "TCR dynamics in human mature T lymphocytes lacking CD3 gamma." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 5947-55, (2003) (PubMed).

Delgado, Fernández, Dave, Kappes, Alarcón: "CD3delta couples T-cell receptor signalling to ERK activation and thymocyte positive selection." in: Nature, Vol. 406, Issue 6794, pp. 426-30, (2000) (PubMed).

Alarcón, Ley, Sánchez-Madrid, Blumberg, Ju, Fresno, Terhorst: "The CD3-gamma and CD3-delta subunits of the T cell antigen receptor can be expressed within distinct functional TCR/CD3 complexes." in: The EMBO journal, Vol. 10, Issue 4, pp. 903-12, (1991) (PubMed).

Details zu CD3 epsilon

Target: CD3 epsilon (CD3E)

Andere Bezeichnung: CD3 epsilon (CD3E Produkte)

Hintergrund: CD3 antigen, epsilon polypeptide,CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.,CD3 epsilon chain, T3E, IMD18

Gen-ID: 916

UniProt: P07766

Pathways: T-Zell Rezeptor Signalweg, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway