DNAJB1 Antikörper

Produktnummer ABIN453940

Dieser Maus Monoklonal Antikörper detektiert spezifisch DNAJB1 in WB, IP, IF, EIA und IHC (fro). Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für DNAJB1 Antikörper (ABIN453940)

Target: DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser DNAJB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 3B9-E6

Produktdetails anti-DNAJB1 Antikörper

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse, Rat

Produktmerkmale: Synonyms: Heat shock 40 kDa protein 1, HSP-40, DnaJ protein homolog 1, HDJ-1, DNAJ1, HDJ1, HSPF1,DnaJ homolog subfamily B member 1

Aufreinigung: Protein G affinity chromatography

Immunogen: Recombinant protein HSP40 (HDJ1)

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blot. Immunoprecipitation. Immunohistochemistry on frozen sections. ELISA. Immunflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS pH 7.2, 50 % glycerol, 0.09 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing andthawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu DNAJB1

Target: DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))

Andere Bezeichnung: DNAJB1 / HSP40

Hintergrund: Human Hsp40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). Members of the Hsp40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. In particular, Hdj1, the first human Hsp40/DnaJ protein identified, plays an important role in protein translation and folding, as well as in the regulation of Hsp70 function (3). HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (4, 5). The Hsp40-Hsp70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (6). Some studies have shown that the difference between HDJ1 and type 1 DNAJ proteins including HDJ2 and yeast YdjI is the result of the possession of a zinc finger domain by the latter, which helps in the function of protein folding. (7, 8).Synonyms: DNAJ1, DnaJ homolog subfamily B member 1, DnaJ protein homolog 1, HDJ-1, HDJ1, HSP-40, HSPF1, Heat shock 40 kDa protein 1

Gen-ID: 3337

UniProt: P25685