HCV NS4 Antikörper

Produktnummer ABIN452528

Produktdetails anti-HCV NS4 Antikörper

Target: HCV NS4 (Hepatitis C Virus NS4 (HCV NS4))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HCV NS4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Produktmerkmale: Synonyms: Hepatitis C Virus, Non-structural protein NS4A, NS4B

Aufreinigung: Affinity Chromatography on Protein G.

Immunogen: Synthetic polypeptide in which overlapping sequences of at least 90 residues in lengthwere selected from the immunodominant NS-4 regions of Chinese HCV strains.

Klon: 5D4-10E7

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA: React with Human Hepatitis C Virus. Western Blot: Use of HCV NS4 antibody (clone 5D4/10E7) at 0.5 μg/mL will allowvisualization of 0.5 μg/lane of synthetic NS-4 peptide and 0.1 μg/lane recombinantChimeric HCV polyprotein. Immunohistochemistry: Immunoperoxidase-Avidin-Biotin (ABC) assay for Formalin-fixedparaffin embedded tissue section using diluted MAb.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with Double distillated water to adjust the final concentration to 1.0 mg/mL.

Buffer: 0.01 M PBS, pH 7.0 without preservatives.

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu HCV NS4

Target: HCV NS4 (Hepatitis C Virus NS4 (HCV NS4))

Andere Bezeichnung: HCV NS4 (HCV NS4 Produkte)

Substanzklasse: Viral Protein

Hintergrund: NS4 A/B are two of the seven nonstructural (NS) proteins making up the HCV polyprotein. The NS proteins recruit the viral genome into an RNA replication complex, which is associated with rearranged cytoplasmic membranes. NS4A acts as a cofactor with the NS3 serine protease and stabilizes its folding. The NS3-NS4A complex is essential for the activation of the latter and allows membrane anchorage of NS3. HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. HCV is responsible for a large proportion of worldwide chronic viral hepatitides. Most of these infections develop into chronic hepatitis, which often progresses to liver cirrhosis and hepatocellular carcinoma. At present, (unlike hepatitis A and B), there is no vaccine to prevent hepatitis C infection. The hepatitis C virus (HCV) nonstructural protein 4B (NS4B) is a relatively hydrophobic 27- kDa protein. The 4A protein has a molecular weight of 6 kDa.Synonyms: Hepatitis C Virus, NS4B, Non-structural protein NS4A