Albumin Antikörper (Biotin)

Produktnummer ABIN401768

Der Kaninchen Polyklonal anti-Albumin Antikörper wird verwendet zum Nachweis von Albumin in Proben von Human. Er wurde validiert für WB, EIA und IHC (fro).

Kurzübersicht für Albumin Antikörper (Biotin) (ABIN401768)

Target: Albumin (ALB)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Albumin Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-Albumin Antikörper (Biotin)

Spezifität: This antibody reacts to Serum Albumin.

Produktmerkmale: Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule

Aufreinigung: Affinity chromatography on Protein A

Immunogen: Human Serum Albumin

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/1,000 - 1/5,000. ELISA: 1/170,000. Immunohistochemistry: 1/500 - 1/3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1.0 mL of deionized water (or equivalent). For extended storage add glycerol to 50 %

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL Bovine Serum Albumin (BSA) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at -20 °C. After reconstitution, store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Albumin

Target: Albumin (ALB)

Andere Bezeichnung: Albumin

Hintergrund: Albumin is a soluble, monomeric protein which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.Synonyms: ALB, BSA, HSA, Serum Albumin

Gen-ID: 213

NCBI Accession: NP_000468

UniProt: P02768

Pathways: Lipid Metabolism