TNKS Antikörper (N-Term)

Produktnummer ABIN401535

Der Ziege Polyklonal anti-TNKS Antikörper (ABIN401535) detektiert spezifisch TNKS in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für TNKS Antikörper (N-Term) (ABIN401535)

Target: TNKS (Tankyrase, TRF1-Interacting Ankyrin-Related ADP-Ribose Polymerase (TNKS))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser TNKS Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-TNKS Antikörper

Bindungsspezifität: N-Term

Spezifität: This antibody detects Tankyrase-1 (TANK1) at N-term. No cross reactivity expected to TANK2.

Kreuzreaktivität (Details): Species reactivity (tested):Human

Aufreinigung: Ammonium sulphate precipitation followed by affinity chromatography using Protein G

Immunogen: Fusion protein containing 120aa amino acids from N Terminal region of the protein sequence according to NP_003738.2 . Genename: TNKS

Anwendungsinformationen

Applikationshinweise: Western blot: In transfected HEK293 transiently expressing TANK1 a band of approx.142 kDa is observed. This band is not observed in the non-transfected HEK293. Thecalculated molecular size is 142 kDa according to NP_003738.2. Recommendedconcentration, 0.5-1.5 μg/mL. Full caption )see picture below): HEK293 overexpressing TANK1 (lane 2) and TANK2 (lane 4)and probed with EB02508 (mock transfection in first lane). Lane three is empty. Lower

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Details zu TNKS

Target: TNKS (Tankyrase, TRF1-Interacting Ankyrin-Related ADP-Ribose Polymerase (TNKS))

Andere Bezeichnung: Tankyrase-1 (TANK1)

Hintergrund: Tankyrase is a protein with homology to ankyrins as well as to the catalytic domain of poly (adenosine diphosphate ribose) polymerase (PARP). Tankyrase localizes to telomeres by binding to the telomeric repeat binding factor 1 (TRF1) through its ankyrin repeats. TRF1 is a negative regulator of telomere length maintenance. Tankyrase exhibits PARP activity functioning as acceptors for adenosine diphosphate (ADP) ribosylation. Since ADP ribosylation of TRF1 diminishes its ability to bind to telomeric DNA, this suggests that telomere function in human cells is regulated by poly (ADP) ribosylation. Both the cell cycle and TRF1 may regulate the subcellular localization of tankyrase. During interphase tankyrase colocalizes with TRF1 to telomeres plus nuclear pore complexes whereas during mitosis tankyrase localizes around the pericentriolar matrix of mitotic chromosomes. Tankyrase may regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4 vesicles.Synonyms: PARP5A, PARPL, TIN1, TINF1, TNKS, TNKS-1, TNKS1, TRF1-interacting ankyrin-related ADP-ribose polymerase, Tankyrase I

Gen-ID: 8658

NCBI Accession: NP_003738

UniProt: O95271