SNX26 Antikörper (C-Term)

Produktnummer ABIN401527

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch SNX26 in EIA und IHC (p). Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für SNX26 Antikörper (C-Term) (ABIN401527)

Target: SNX26 (Sorting Nexin 26 (SNX26))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser SNX26 Antikörper ist unkonjugiert

Applikation: Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-SNX26 Antikörper

Bindungsspezifität: C-Term

Sequenz: SWSLHSEGQT RSYC

Spezifität: This antibody detects SNX26 at C-term.

Kreuzreaktivität (Details): Species reactivity (expected):MouseSpecies reactivity (tested):Human

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the C Terminus of the protein sequence according to NP_443180.2. Genename: SNX26

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1: 8000. Western blot: Preliminary experiments gave an approx 75 kDa band in Human Bone Marrow,Kidney, and Pancreas lysates after 1 μg/mL antibody staining. ABIN401527 may detect theisoform A1A5D2 (UniProtKB) with a molecular weight of 81.5 kDa which is similar in aminoacid sequence to NP_443180.2 however lacking aa729-1268. Please note that currently wecannot find a precise explanation in the literature for the band we observe given thecalculated size of 125 kDa according to NP_443180.2. The 75 kDa band was successfullyblocked by incubation with the immunizing peptide. We would appreciate any feedbackfrom people in the field - have any results been reported with other antibodies/lysates?Have any further splice variants/modified forms been reported?Immunohistochemistry on paraffin sections: 2.5 μg/mL. In Human Brain shows cytoplasmstaining of Purkinje cells in Cerebellum.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Details zu SNX26

Target: SNX26 (Sorting Nexin 26 (SNX26))

Andere Bezeichnung: Sorting Nexin-26 (SNX26)

Hintergrund: SNX26 may be involved in several stages of intracellular trafficking. It could play an important role in the regulation of glucose transport by insulin. It may act as a downstream effector of RHOQ/TC10 in the regulation of insulin-stimulated glucose transport.Synonyms: ARHGAP33, Rho GTPase-activating protein 33, TCGAP

Gen-ID: 115703

NCBI Accession: NP_443180

UniProt: O14559