SMAD1 Antikörper (Ser206)

Produktnummer ABIN401471

Produktdetails anti-SMAD1 Antikörper

Target: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Bindungsspezifität: Ser206

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Huhn, Pferd, Drosophila melanogaster, Schaf, Schwein, Bonobo, Schimpanse, Schnabeltier (Platypus), Gorilla, Makake, Opossum, Orang-Utan, Salmon

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMAD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Spezifität: This antibody is directed against SMAD1 protein.

Kreuzreaktivität (Details): Species reactivity (expected):Duckbill platypus, Drosophila, Mouse, Opossum, Chimpanzee, Bonobo, Orangutan, Gorilla, Macaque, Cattle, Sheep, Porcine, Rat, Horse, Salmon, Chicken, Canine.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Immunogen: Synthetic peptide corresponding to the region of amino acids containing serine 206 of human SMAD1 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/25,000 - 1/30,000. Western Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.31 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu SMAD1

Target: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Andere Bezeichnung: SMAD1 (SMAD1 Produkte)

Synonyme: MLP antikoerper, Xmad1 antikoerper, Xsmad1 antikoerper, bsp1 antikoerper, jv4-1 antikoerper, jv41 antikoerper, madh1 antikoerper, madr1 antikoerper, smad-1 antikoerper, MADH1 antikoerper, fb39h09 antikoerper, wu:fb39h09 antikoerper, wu:fb97e04 antikoerper, 2/23 antikoerper, CG12399 antikoerper, Dmel\\CG12399 antikoerper, E(zen)2 antikoerper, En(vvl) antikoerper, MAD antikoerper, Mat antikoerper, P-Mad antikoerper, P-mad antikoerper, PMad antikoerper, Smad antikoerper, Smad1 antikoerper, apg antikoerper, c28 antikoerper, dMAD antikoerper, dMad antikoerper, l(2)K00237 antikoerper, l(2)k00237 antikoerper, mad antikoerper, p-Mad antikoerper, pMAD antikoerper, pMad antikoerper, pSmad antikoerper, BSP-1 antikoerper, BSP1 antikoerper, JV4-1 antikoerper, JV41 antikoerper, MADR1 antikoerper, AI528653 antikoerper, Madh1 antikoerper, Madr1 antikoerper, Mlp1 antikoerper, MusMLP antikoerper, smad1 antikoerper, xmad antikoerper, SMAD family member 1 antikoerper, Mothers against dpp antikoerper, SMAD family member 1 L homeolog antikoerper, smad1 antikoerper, SMAD1 antikoerper, Mad antikoerper, Smad1 antikoerper, smad1.L antikoerper

Hintergrund: SMAD1 is also known Mothers Against Decapentaplegic homolog 1, Mothers against DPP homolog 1, hSMAD-3, JV4-1, Transforming growth factor-Beta-Signaling Protein 1 or BSP1. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD1, as a transcriptional modulator, is activated by BMP (Bone Morphogenetic Protein) type 1 receptor kinase (it is a receptor-regulated SMAD or R-SMAD). BMPs are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. SMAD proteins have been implicated as downstream effectors of TGF beta/BMP signaling. In response to BMP ligands, SMAD1 can be phosphorylated (other sites besides the most prominent of S206, are S187, S195, and S214). S-206 is phosphorylated by ERK in response to mitogenic growth factors, or by recombinant ERK in vitro, this can be tested by treating cells with EGF or in cancer cells where Ras is activated. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is also a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasomemediated degradation.Synonyms: BSP-1, BSP1, JV4-1, MAD homolog 1, MADH1, MADR1, Mad-related protein 1, Mothers against DPP homolog 1, Mothers against decapentaplegic homolog 1, SMAD 1, SMAD family member 1, SMAD-1, Transforming growth factor-beta-signaling protein 1

Gen-ID: 4086

NCBI Accession: NP_001003688

UniProt: Q15797

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development