RNF25 Antikörper (C-Term)

Produktnummer ABIN401373

Der Kaninchen Polyklonal anti-RNF25 Antikörper (ABIN401373) detektiert spezifisch RNF25 in WB, IP und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für RNF25 Antikörper (C-Term) (ABIN401373)

Target: RNF25 (Ring Finger Protein 25 (RNF25))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RNF25 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Produktdetails anti-RNF25 Antikörper

Bindungsspezifität: C-Term

Spezifität: This antibody is directed against RNF25 protein.

Aufreinigung: Affinity chromatography

Immunogen: Synthetic peptide corresponding to amino acids near the carboxyl terminus of human RNF25 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/10,000 - 1/40,000. Western Blot: 1/200 - 1/2,000. Immunoprecipitation: 1 μg.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu RNF25

Target: RNF25 (Ring Finger Protein 25 (RNF25))

Andere Bezeichnung: RNF25

Hintergrund: RING finger protein 25 (RNF25, also named AO7) contains a RING finger domain and is ubiquitously expressed in various tissues. RNF25 was initially identified in a yeast two-hybrid screen of a murine T-cell library by using UbcH5b, an E2 enzyme, as bait. RNF25 has also been shown to act as a putative E3 ligase, at least in vitro. RNF25 localizes predominantly in the nucleus and supports the transcriptional activity of NF-kappaB by interacting with p65 in vivo upon stimulation with TNF. Yeast two-hybrid data also suggest that RNF25 interacts with a number of other molecules which may be potential ubiquitin ligase substrates. Among these are molecules that have critical roles in signal transduction and in regulation of translation (personal communication, Allan Weissman, CCR-NCI, Bethesda, MD).Synonyms: E3 ubiquitin-protein ligase RNF25, RING finger protein 25

Gen-ID: 64320

NCBI Accession: NP_071898

UniProt: Q96BH1