TUBA1B Antikörper (AA 427-441)

Produktnummer ABIN401310

Dieses Anti-TUBA1B-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von TUBA1B in WB und EIA. Geeignet für Human, Avian und Säugetier.

Kurzübersicht für TUBA1B Antikörper (AA 427-441) (ABIN401310)

Target: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Reaktivität: Human, Avian, Säugetier

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TUBA1B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-TUBA1B Antikörper

Bindungsspezifität: AA 427-441

Spezifität: This antibody is directed against alpha Tubulin protein. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent Loading Control.

Aufreinigung: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to amino acids 427-441 of Human alpha Tubulin

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/5,000-1/25,000. Immunofluorescence: 1/500-1/2,000Western Blot: 1/500-1/3,000. Expect a band at approx. 50 kDa in size corresponding to alpha tubulin by Western blotting inmost cell lysates or extracts.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.08 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu TUBA1B

Target: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Andere Bezeichnung: alpha Tubulin / TUBA1B

Hintergrund: Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle.Synonyms: Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain

Gen-ID: 10376

NCBI Accession: NP_006073

UniProt: P68363

Pathways: Microtubule Dynamics, M Phase