LIM Domain Kinase 1 Antikörper (AA 630-647)

Produktnummer ABIN401293

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch LIM Domain Kinase 1 in WB und EIA. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Schimpanse und Orang-Utan.

Kurzübersicht für LIM Domain Kinase 1 Antikörper (AA 630-647) (ABIN401293)

Target: LIM Domain Kinase 1 (LIMK1)

Reaktivität: Human, Maus, Ratte, Schimpanse, Orang-Utan

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LIM Domain Kinase 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-LIM Domain Kinase 1 Antikörper

Bindungsspezifität: AA 630-647

Spezifität: This antibody reacts to LIM kinase protein.

Kreuzreaktivität (Details): Species reactivity (expected):Chimpanzee, Orangutan, Mouse, Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity Purified

Immunogen: Synthetic peptide corresponding aa 630-647 of human LIM kinase protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/8000 - 1/36000. Western Blot: 1/500 - 1/2000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu LIM Domain Kinase 1

Target: LIM Domain Kinase 1 (LIMK1)

Andere Bezeichnung: LIMK1

Hintergrund: LIM Kinase is also known as LIM-domain containing protein kinase, LIMK-1 and LIMK. There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase 1 and LIM kinase 2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Two splice variant have been identified. LIM kinase has a cytoplamic localization.Synonyms: EC=2.7.11.1, LIM domain kinase 1, LIMK, LIMK-1

Gen-ID: 3984

NCBI Accession: NP_001191355

UniProt: P53667

Pathways: Caspase Kaskade in der Apoptose, Regulation of Cell Size, CXCR4-mediated Signaling Events