SLIT2 Antikörper (AA 484-500)

Produktnummer ABIN401286

Der Kaninchen Polyklonal anti-SLIT2 Antikörper (ABIN401286) detektiert spezifisch SLIT2 in WB und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte, Huhn, Rind (Kuh), Tetraodon nigroviridis und Xenopus laevis.

Kurzübersicht für SLIT2 Antikörper (AA 484-500) (ABIN401286)

Target: SLIT2 (Slit Homolog 2 (Drosophila) (SLIT2))

Reaktivität: Human, Maus, Ratte, Huhn, Rind (Kuh), Tetraodon nigroviridis, Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SLIT2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-SLIT2 Antikörper

Bindungsspezifität: AA 484-500

Spezifität: This antibody is directed against SLIT-2 protein.

Kreuzreaktivität (Details): Species reactivity (expected):Chicken, Mouse, Rat, Bovine, Xenopus laevis.
Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography

Immunogen: Synthetic peptide corresponding aa 484-500 of Human SLIT-2 protein.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/1,500 - 1/6,000. Western Blot: 1/500 - 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted 2-8 °C fo one month or (in aliquots) at-20 °C for longer.

Details zu SLIT2

Target: SLIT2 (Slit Homolog 2 (Drosophila) (SLIT2))

Andere Bezeichnung: SLIT2

Hintergrund: SLIT-2 is thought to act as a molecular guidance cue in cellular migration, and its function appears to be mediated by interaction with roundabout homologue receptors. SLIT-1 and SLIT-2 seem to be essential for midline guidance in the forebrain by acting as repulsive signals preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development SLIT-2 may play role in guiding commissural axons once they reach the floor plate by modulating the response to netrin. In vitro, SLIT-2 silences the attractive effect of NTN1 but not its growthstimulatory effect and silencing requires the formation of a ROBO1-DCC complex. In vitro, only commissural axons that crossed the midline responded to SLIT-2. In the developing visual system this protein functions as a repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. SLIT-2 is a secreted protein predominantly expressed in fetal lung and kidney, and adult spinal cord. Weak expression is observed in adult adrenal gland, thyroid, trachea and other tissues examined. Multiple isoforms have been reported for this product.Synonyms: SLIL3, SLIT-2, Slit homolog 2 protein

Gen-ID: 9353

NCBI Accession: NP_004778

UniProt: O94813

Pathways: Regulation of Actin Filament Polymerization, Regulation of Cell Size, Smooth Muscle Cell Migration