USP7 Antikörper (N-Term)

Produktnummer ABIN401255

Der Kaninchen Polyklonal anti-USP7 Antikörper wird verwendet zum Nachweis von USP7 in Proben von Human, Maus, Ratte, Hund, Rind (Kuh) und Schimpanse. Er wurde validiert für WB und EIA.

Kurzübersicht für USP7 Antikörper (N-Term) (ABIN401255)

Target: USP7 (Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7))

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh), Schimpanse

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser USP7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-USP7 Antikörper

Bindungsspezifität: N-Term

Spezifität: This affinity purified antibody is directed against HAUSP protein.

Kreuzreaktivität (Details): Species reactivity (expected):Rat, Mouse, Chimpanzee, Bovine and Dog (100 %).Chicken (92 %, 11/12).Zebrafish (83 %, 10/12).
Species reactivity (tested):Human.

Aufreinigung: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to amino acids near the amino terminus of Human HAUSP protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/15,000-1/60,000. Western blot: 1/500-1/2,000 (Expect a band approximately 130 kDa in size correspondingto HAUSP protein in the appropriate cell lysate or extract).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.98 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or for longer (in aliquots) at-20 °C or below.

Details zu USP7

Target: USP7 (Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7))

Andere Bezeichnung: USP7 / HAUSP

Hintergrund: HAUSP (also known as deubiquitinating enzyme 7, herpes virus associated ubiquitin specific protease, TEF1, ubiquitin carboxyl terminal hydrolase 7, ubiquitin specific protease 7, ubiquitin thiolesterase 7, and USP7) is a novel p53 interacting protein. HAUSP was identified by mass spectrometry of affinity purified p53 associated factors by Li et al. HAUSP strongly stabilizes p53, even in the presence of excess MDM2, and also induces p53- dependent cell growth repression and apoptosis. HAUSP has an intrinsic enzymatic activity that specifically deubiquitinates p53 both in vivo and in vitro. Expression of a catalytically inactive point mutation of HAUSP in cells increased the levels of p53 ubiquitination and also destabilized p53. Li et al concluded that their findings revealed an important mechanism by which p53 can be stabilized by direct de-ubiquitination and also implied that HAUSP may function as a tumor suppressor in vivo through the stabilization of p53.Synonyms: Deubiquitinating enzyme 7, Herpesvirus-associated ubiquitin-specific protease, Ubiquitin carboxyl-terminal hydrolase 7, Ubiquitin thioesterase 7, Ubiquitin-specific-processing protease 7

Gen-ID: 7874

NCBI Accession: NP_003461

UniProt: Q93009