beta Amyloid Antikörper (Amyloid beta) (AA 1-14)

Details for Product anti-Abeta Antibody No. ABIN401205
Antigen
  • AAA
  • ABETA
  • ABPP
  • AD1
  • APPI
  • CTFgamma
  • CVAP
  • PN-II
  • PN2
  • aaa
  • abeta
  • abpp
  • ad1
  • appi
  • ctfgamma
  • cvap
  • pn2
  • Abeta
  • Abpp
  • Adap
  • Ag
  • Cvap
  • E030013M08Rik
  • betaApp
  • amyloid beta precursor protein
  • amyloid beta (A4) precursor protein
  • APP
  • app
  • App
Epitop
AA 1-14, N-Term
51
35
20
10
10
7
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus
157
38
27
9
6
5
5
4
3
2
2
1
1
1
1
1
1
Wirt
Kaninchen
100
65
6
2
Klonalität
Polyklonal
Konjugat
Dieser beta Amyloid Antikörper ist unkonjugiert
11
4
2
1
1
1
Applikation
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
115
97
58
37
35
28
17
16
11
5
5
3
2
1
1
1
1
1
1
1
1
Optionen
Immunogen Synthetic peptide corresponding to the amino terminus (1-14) of Human beta amyloid conjugated to KLH using maleimide.
Sequenz D-A-E-F-R-H-D-S-G-Y-E-V-H-H
Isotyp IgG
Kreuzreaktivität (Details) Species reactivity (tested):Human, Mouse.
Reinigung Affinity Chromatography
Antigen
Andere Bezeichnung Amyloid beta (Abeta Antibody Abstract)
Hintergrund Beta amyloid, often abbreviated as A-beta, is a protein that builds up in the brains of persons with Alzheimer's disease, collecting in clumps called plaques or senile plaques. While some researchers question whether beta amyloid is the cause of the dementia, most agree that it is involved in the disruption of thinking that is a hallmark of the disease. In some cases of familial Alzheimer's disease, mutations in genes for the proteins called the presenilins lead to increased production of amyloid. Researchers have been looking at how presenilin-1 in particular contributes to the excess buildup of beta amyloid. Presenilin-1 apparently acts to increase the activity of gamma-secretase, an enzyme that changes a normal protein (amyloid precursor protein or APP) into beta amyloid itself. Furthermore, presenilin-1 might be gamma-secretase.Synonyms: Amyloid beta peptide
Applikations-hinweise ELISA: 1/10,000. Western blot: 0.5-1.0 μg/mL. A 40-50 kD band consistent with a higher MW precursor isdetected in using whole tissue extracts from mouse brain. Immunofluorescence. Immunohistochemistry: We recommend the use of 4 % PFA for Paraffin embedded tissuesand 10 % Formalin for Frozen tissue for fixation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 0.55 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or(in aliquots) at-20 °C or below for longer .
Bilder des Herstellers
Image no. 1 for anti-Amyloid beta (Abeta) (AA 1-14), (N-Term) antibody (ABIN401205) anti-Amyloid beta (Abeta) (AA 1-14), (N-Term) antibody (Image 1)
Image no. 2 for anti-Amyloid beta (Abeta) (AA 1-14), (N-Term) antibody (ABIN401205) anti-Amyloid beta (Abeta) (AA 1-14), (N-Term) antibody (Image 2)
Image no. 3 for anti-Amyloid beta (Abeta) (AA 1-14), (N-Term) antibody (ABIN401205) anti-Amyloid beta (Abeta) (AA 1-14), (N-Term) antibody (Image 3)
Allgemeine Veröffentlichungen Frenkel, Balass, Solomon: "N-terminal EFRH sequence of Alzheimer's beta-amyloid peptide represents the epitope of its anti-aggregating antibodies." in: Journal of neuroimmunology, Vol. 88, Issue 1-2, pp. 85-90, 1998 (PubMed).

Suo, Tan, Placzek, Crawford, Fang, Mullan: "Alzheimer's beta-amyloid peptides induce inflammatory cascade in human vascular cells: the roles of cytokines and CD40." in: Brain research, Vol. 807, Issue 1-2, pp. 110-7, 1998 (PubMed).

Bonaiuto, McDonald, Rossi, Cassatella: "Activation of nuclear factor-kappa B by beta-amyloid peptides and interferon-gamma in murine microglia." in: Journal of neuroimmunology, Vol. 77, Issue 1, pp. 51-6, 1997 (PubMed).

Paradis, Douillard, Koutroumanis, Goodyer, LeBlanc: "Amyloid beta peptide of Alzheimer's disease downregulates Bcl-2 and upregulates bax expression in human neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 16, Issue 23, pp. 7533-9, 1997 (PubMed).

Hoshi, Takashima, Murayama, Yasutake, Yoshida, Ishiguro, Hoshino, Imahori: "Nontoxic amyloid beta peptide 1-42 suppresses acetylcholine synthesis. Possible role in cholinergic dysfunction in Alzheimer's disease." in: The Journal of biological chemistry, Vol. 272, Issue 4, pp. 2038-41, 1997 (PubMed).

Kaltschmidt, Uherek, Volk, Baeuerle, Kaltschmidt: "Transcription factor NF-kappaB is activated in primary neurons by amyloid beta peptides and in neurons surrounding early plaques from patients with Alzheimer disease." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 6, pp. 2642-7, 1997 (PubMed).

Solomon, Koppel, Frankel, Hanan-Aharon: "Disaggregation of Alzheimer beta-amyloid by site-directed mAb." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 8, pp. 4109-12, 1997 (PubMed).

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