beta Amyloid Antikörper (N-Term)

Produktnummer ABIN401205

Dieser Anti-beta Amyloid Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von beta Amyloid in WB, IF, EIA und IHC (p). Geeignet für Human und Maus.

Kurzübersicht für beta Amyloid Antikörper (N-Term) (ABIN401205)

Target: beta Amyloid (Abeta) (Amyloid beta (Abeta))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser beta Amyloid Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-beta Amyloid Antikörper

Bindungsspezifität: AA 1-14, N-Term

Sequenz: D-A-E-F-R-H-D-S-G-Y-E-V-H-H

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse.

Aufreinigung: Affinity Chromatography

Immunogen: Synthetic peptide corresponding to the amino terminus (1-14) of Human beta amyloid conjugated to KLH using maleimide.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/10,000. Western blot: 0.5-1.0 μg/mL. A 40-50 kD band consistent with a higher MW precursor isdetected in using whole tissue extracts from mouse brain. Immunofluorescence. Immunohistochemistry: We recommend the use of 4 % PFA for Paraffin embedded tissuesand 10 % Formalin for Frozen tissue for fixation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.55 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or(in aliquots) at-20 °C or below for longer .

Details zu beta Amyloid

Target: beta Amyloid (Abeta) (Amyloid beta (Abeta))

Andere Bezeichnung: Amyloid beta

Hintergrund: Beta amyloid, often abbreviated as A-beta, is a protein that builds up in the brains of persons with Alzheimer's disease, collecting in clumps called plaques or senile plaques. While some researchers question whether beta amyloid is the cause of the dementia, most agree that it is involved in the disruption of thinking that is a hallmark of the disease. In some cases of familial Alzheimer's disease, mutations in genes for the proteins called the presenilins lead to increased production of amyloid. Researchers have been looking at how presenilin-1 in particular contributes to the excess buildup of beta amyloid. Presenilin-1 apparently acts to increase the activity of gamma-secretase, an enzyme that changes a normal protein (amyloid precursor protein or APP) into beta amyloid itself. Furthermore, presenilin-1 might be gamma-secretase.Synonyms: Amyloid beta peptide

Pathways: Inflammasome