IL27 Antikörper (HRP)

Produktnummer ABIN401111

Der Kaninchen Polyklonal Anti-IL27-Antikörper wurde für WB und EIA validiert. Er ist geeignet, IL27 in Proben von Maus zu detektieren.

Kurzübersicht für IL27 Antikörper (HRP) (ABIN401111)

Target: IL27 (Interleukin-27 Subunit alpha (IL27))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL27 Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-IL27 Antikörper (HRP)

Spezifität: This antibody reacts to Interleukin-27A.

Kreuzreaktivität (Details): Species reactivity (expected):Rat.
Species reactivity (tested):Mouse.

Aufreinigung: Ion exchange chromatography

Immunogen: Full length recombinant mouse IL27/p28 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/10,000 - 1/50,000. Western Blot: 1/1,000 - 1/5,000. Immunohistochemistry: 1/500 - 1/2,500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.1 mL of deionized water (or equivalent).

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL Bovine Serum Albumin as stabilizer and 0.01 % (w/v) Gentamicin Sulfate as preservative (Do NOT add Sodium Azide!)

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu IL27

Target: IL27 (Interleukin-27 Subunit alpha (IL27))

Andere Bezeichnung: Interleukin-27 / IL27A

Hintergrund: The cytokine Interleukin 27 (IL-27) is produced in response to inflammation. It is made by activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells. IL-27 consists of a heterodimeric combination of Epstein-Barr virus-induced molecule 3 (EBI3, or IL-27B) non-covalently linked with IL-27 p28 (or IL-27A). It is a regulator of T helper cell development and suppressor of T-cell proliferation. IL-27 has both pro- and anti-inflammatory properties. It can stimulate cytotoxic T cell activity and induce isotype switching in B-cells. It has diverse effects on innate immune cells. It induces monocytes and mast cells to secrete pro-inflammatory cytokines. When infection is present, IL-27 induces naive CD4+ T cells to proliferate and develop Th1 cell responses. As an anti-inflammatory regulator, IL-27 can inhibit Th1 or Th2 responses and restrict the strength and duration of adaptive immune responses. The IL-27 p28 subunit, a 28 kDa glycoprotein belonging to the type I cytokine family, is homologous to IL-12 p35, IL-23 p19, and IL-6. The EBI3 (Epstein-Barr virus-induced molecule 3, or IL-27B) subunit is a 34 kDa glycoprotein containing two fibronectin type III domains, and belongs to the type I cytokine receptor family. It can exist as a homodimer and can also heterodimerize with IL-12 p35. It is homologous to the p40 subunit of IL-12 and IL-23 and to the extracellular domain of IL-6 R. EBI3 can heterodimerize also with IL-12 p35, or can exist as a homodimer. The heterodimeric IL-27 receptor contains WSX-1 (TCCR) and gp130. WSX-1 is specific for IL-27, and is expressed on resting/naive CD4+ T cells, CD8+ T cells, NK cells, dendritic cells, monocytes, mast cells, and B cells. Gp130, on the other hand, functions as a subunit of the receptor complexes for at least seven other cytokines. IL-27 also promotes effector functions of NK cells and CD8+ T cells.Synonyms: IL-27 subunit alpha, IL-30, IL27-A, IL30, Interleukin-30, p28

Gen-ID: 246779

NCBI Accession: NP_663611

UniProt: Q8K3I6