S100A1 Antikörper

Produktnummer ABIN401026

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch S100A1 in WB, IHC (p) und EIA. Er zeigt eine Reaktivität gegenüber Rind (Kuh).

Kurzübersicht für S100A1 Antikörper (ABIN401026)

Target: S100A1 (S100 Calcium Binding Protein A1 (S100A1))

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser S100A1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-S100A1 Antikörper

Spezifität: This antibody is directed against S100 protein.

Kreuzreaktivität (Details): Species reactivity (expected):Primate, Mouse, Rat, Canine.
Species reactivity (tested):Bovine.

Aufreinigung: Affinity chromatography on Protein A

Immunogen: Prepared from whole rabbit serum produced by repeated immunizations with fulllength bovine S100 protein (mixture of alphaalpha homodimers and alphabeta heterodimers)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/5,000 - 1/25,000. Western Blot: 1/500 - 1/3,000. Immunohistochemistry: 1/200 - 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.1 mL of deionized water (or equivalent).

Konzentration: 5.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu S100A1

Target: S100A1 (S100 Calcium Binding Protein A1 (S100A1))

Andere Bezeichnung: S100A1

Hintergrund: S-100 protein derived from brain tissue is an acidic calcium-binding protein with molecular weight of about 21 kDa. In human brain tissue S-100 protein is mainly presented as two isoforms - betabeta homodimer (S-100b) and alphabeta heterodimer (S-100a). Because of its predominant location in astroglial cells S-100 protein can be used as a sensitive and reliable marker for central nervous system injury. Structural damage of glial cells causes leakage of S-100 protein into the extracellular matrix and into cerebrospinal fluid, further releasing into the bloodstream. Measurements of S-100 protein in patient serum samples are useful in monitoring of traumatic brain injury, ischemic brain damage after circulatory arrests, and in diagnosis and prognosis of clinical outcome in acute stroke. Although predominant among the water-soluble brain proteins, S-100 is also found in a variety of other tissues. S-100 is an intracellular protein that weakly binds calcium. It binds zinc very tightly, however, and this appears to increase the affinity of the protein for calcium. Distinct binding sites, with different affinities, exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.Synonyms: S-100 protein alpha chain, S-100 protein subunit alpha, S100 alpha, S100 calcium-binding protein A1, S100-A1, S100A

Gen-ID: 528735

NCBI Accession: NP_001092512

UniProt: P02639

Pathways: Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades, S100 Proteine