JAG1 Antikörper (AA 110-125)

Produktnummer ABIN401024

Der Kaninchen Polyklonal anti-JAG1 Antikörper wird verwendet zum Nachweis von JAG1 in Proben von Human, Maus, Ratte und Schimpanse. Er wurde validiert für WB, IF, IHC (p) und EIA.

Kurzübersicht für JAG1 Antikörper (AA 110-125) (ABIN401024)

Target: JAG1 (Jagged 1 (JAG1))

Reaktivität: Human, Maus, Ratte, Schimpanse

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser JAG1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-JAG1 Antikörper

Bindungsspezifität: AA 110-125

Spezifität: This protein A purified antibody is directed against Human Jagged-1 protein. Expect a band approximately 150 kDa in size corresponding to Jagged-1 in Mouse liver whole cell lysates, and a 75 kDa band in Human brain and kidney lysates. A BLAST analysis was used to suggest cross reactivity with Jagged-1 protein from Human, Chimpanzee, Rat and Mouse based on 100 % homology with the immunizing sequence. Partial reactivity is expected against dog (81 %) and Xenopus laevis (85 %) based on partial sequence homologies as indicated. Reactivity against homologues from other sources is not known.

Kreuzreaktivität (Details): Species reactivity (expected):Chimpanzee, Rat.
Species reactivity (tested):Human and Mouse.

Aufreinigung: Affinity Chromatography on Protein A.

Immunogen: Synthetic peptide corresponding to amino acids 110-125 of Human Jagged-1 protein.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suitable for use in ELISA, Immunohistochemistry, Immunofluorescence microscopy andWestern blot. Recommended Dilutions: ELISA: 1/20,000-1/100,000. Western Blot: 1/500-1/2,000. Immunofluorescence: 1/200-1/1,000. Immunohistochemistry on Paraffin Sections: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at-20 °C.

Details zu JAG1

Target: JAG1 (Jagged 1 (JAG1))

Andere Bezeichnung: CD339 / JAG1

Hintergrund: Jagged 1 protein encoded by the JAG1 gene, that is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the Jagged 1 protein cause Alagille syndrome. Jagged 1 signaling through notch 1 has also been shown to play a role in hematopoies.Synonyms: JAGL1, Jagged-1, hJ1

Gen-ID: 182

NCBI Accession: NP_000205

UniProt: P78504

Pathways: Notch Signalweg, Stem Cell Maintenance