PDCD4 Antikörper (pSer457)

Produktnummer ABIN400797

Dieses Maus Monoklonal-Antikörper erkennt spezifisch PDCD4 in WB, IHC (p), EIA und IHC (fro). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte und Xenopus laevis.

Kurzübersicht für PDCD4 Antikörper (pSer457) (ABIN400797)

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Reaktivität: Human, Maus, Ratte, Xenopus laevis

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PDCD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 9G6

Produktdetails anti-PDCD4 Antikörper

Bindungsspezifität: pSer457

Spezifität: This antibody is specific for Pdcd4 protein phosphorylated at Ser457. Expect a band approximately 62 kDa in size corresponding to phosphorylated Pdcd4 protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with Human Pdcd4 pS457 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat and Xenopus (100 %).
Species reactivity (tested):Human.

Produktmerkmale: Synonyms: H731, Programmed cell death protein 4, Neoplastic transformation inhibitor protein,Nuclear antigen H731-like

Aufreinigung: Protein A Chromatography

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 400,000. Western blot: 1: 1,000 - 1: 3,000. Immunohistochemistry on frozen sections: 1.25 - 2.5 μg/mL, we recommend the use of freshfrozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2 °C to 8 °C up to one month or (in aliquots) at -20 °C to -70 °C forlonger. To minimize loss of volume (25μl) dilute 1: 10 by adding 225 μl of the buffer statedabove directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect thevolume at the bottom of the vial. Use this intermediate dilution when calculating finaldilutions. Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu PDCD4

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Andere Bezeichnung: PDCD4

Hintergrund: Programmed cell death 4 (Pdcd4) is a novel tumor suppressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)- responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun.Synonyms: H731, Neoplastic transformation inhibitor protein, Nuclear antigen H731-like, Programmed cell death protein 4

Gen-ID: 27250

UniProt: Q53EL6