PMS2 Antikörper (AA 623-639)

Produktnummer ABIN400707

Der Maus Monoklonal anti-PMS2 Antikörper (Klon 163C1251) (ABIN400707) detektiert spezifisch PMS2 in WB und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für PMS2 Antikörper (AA 623-639) (ABIN400707)

Target: PMS2 (PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PMS2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 163C1251

Produktdetails anti-PMS2 Antikörper

Bindungsspezifität: AA 623-639

Spezifität: Recognizes Postmeiotic Segregation Increased 2 (PMS2).

Kreuzreaktivität (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Produktmerkmale: Synonyms: PMSL2, PMS-2, Mismatch repair endonuclease PMS2, PMS1 protein homolog 2, DNAmismatch repair protein PMS2

Aufreinigung: Protein G Chromatography.

Immunogen: Synthetic peptide (KLH conjugated) corresponding to amino acids 623-639 of HumanPMS2. AA Sequence: SSLAKRIKQLHHEAQQS

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blot: 1-2 μg/mL. Immunohistochemistry on Paraffin Sections: 5 μg/mL. In IHC with FFPE tissues this antibody shows staining of Mucin in Colon in addition to theexpected signal.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu PMS2

Target: PMS2 (PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2))

Andere Bezeichnung: PMS2

Hintergrund: Mismatch DNA repair in bacteria is carried out by the MutL, MutH, and MutS proteins. The MutS protein initially bunds to mismatched DNA. This is followed by binding of the MutH endonuclease and MutL to form a complex that carries out excision repair. The hMSH2 gene specifies a MutS homologue and hMLH1, hPMS1, and hPMS2 encode MutL homologs. Mutations in these genes are associated with hereditary nonpolyposis colon cancer (HNPCC), one of the most common hereditary diseases in man. As with the bacterial system, HNPCC is characterized by frequent microsatellite mutations that arise by somatic mutation due to a replication error (RER+) phenotype. Both hPMS1 and hPMS2 are mutated in the germline of HNPCC patients. Although the exact function of MutL and its homologs has yet to be determined, it is known that a complex of PMS2 and MLH1 (MutLa) from HeLa cells can complement a deficiency of MLH1 in hypermutable H6 colorectal tumor cells.Synonyms: DNA mismatch repair protein PMS2, Mismatch repair endonuclease PMS2, PMS1 protein homolog 2, PMSL2

Gen-ID: 5395

UniProt: P54278

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response