CENPF Antikörper (AA 1759-2093)

Produktnummer ABIN396951

Dieser Anti-CENPF Antikörper ist ein Maus Monoklonal Antikörper zur Detektion von CENPF in IHC, WB, IP, IHC (p) und IHC (fro). Geeignet für Human.

Kurzübersicht für CENPF Antikörper (AA 1759-2093) (ABIN396951)

Target: CENPF (Centromere Protein F (CENPF))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CENPF Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-CENPF Antikörper

Bindungsspezifität: AA 1759-2093

Marke: IHC-plus™

Spezifität: Recognizes mitosin, a 350 kD phosphoprotein that is specifically involved in mitotic-phase progression. Mitosin is expressed throughout S, G2, and M phases of the cell cycle, but is absent in G0 and G1. It associates with the mitotic apparatus during M-phase and is redistributed from the nucleus to the centromere, spindle, and midbody during M-phase progression. Its usefulness as a proliferation marker is under active investigation.

Aufreinigung: Protein G purified

Immunogen: GST fusion protein expressed in E. coli corresponding to aa 1759-2093 of human mitosin.

Type of Immunogen: Fusion protein

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-Fr (10 μg/mL), IHC-P (10 μg/mL), IP (1 - 10 μg/mL), WB (1 - 10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: 1-10 μg/mL. Immunoprecipitation: 1-10 μg/mL. Immunohistochemistry: 10 μg/mL with frozen or paraffin-embedded sections. Tested in IHC using formalin-fixed paraffin-embedded human tissues, HIER antigen retrieval using sodium citrate buffer at  pH 6.0.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4

Handhabung: Avoid freeze/thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -70°C, avoid freeze/thaw cycles.

Details zu CENPF

Target: CENPF (Centromere Protein F (CENPF))

Andere Bezeichnung: CENPF / CENP-F

Hintergrund: Name/Gene ID: CENPF

Synonyms: CENPF, AH antigen, Centromere protein F, CENF, CENP-F, Kinetochore protein CENPF, Mitosin, CENP-F kinetochore protein, Hcp-1, PRO1779

Gen-ID: 1063

UniProt: P49454

Pathways: Chromatin Binding, M Phase, SARS-CoV-2 Protein Interaktom, Phosphorylierungen bei SARS-CoV-2 Infektion