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Vimentin Antikörper (AA 63-90)

Name: Vimentin Antikörper (AA 63-90)
SKU: ABIN389211
Price: 445.5 EUR
Availability: InStock

VIM Reaktivität: Human, Maus WB, IF, IHC (p) Wirt: Kaninchen Polyclonal RB15215 unconjugated

All lanes : Anti-Vimentin Antibody (S82) at 1:1000 dilution Lane 1: 293 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: U-2OS whole cell lysate Lane 4: C2C12 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lysates/proteins at 20 μg per lane. (Vimentin Antikörper (AA 63-90))

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Vimentin Antibody (S82) (ABIN389211 and ABIN2839366) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (Vimentin Antikörper (AA 63-90))

IHC (p)

Immunofluorescent analysis of 4 % paraformaldehyde-fixed, 0. (Vimentin Antikörper (AA 63-90))

Western blot analysis of Vimentin-S82 (ABIN389211 and ABIN2839366) in Hela cell line lysates (35 μg/lane). (Vimentin Antikörper (AA 63-90))

Western blot analysis of VIM(arrow) using rabbit polyclonal Vimentin Antibody (S82) (ABIN389211 and ABIN2839366). (Vimentin Antikörper (AA 63-90))

5 Abbildungen

Produktnummer ABIN389211

Datenblatt (PDF)

Target Alle Vimentin (VIM) Antikörper anzeigen

Vimentin (VIM)
Bindungsspezifität
32

18

16

16

16

14

11

8

8

7

4

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 63-90
Reaktivität
333

127

125

61

59

44

43

37

30

22

20

14

13

8

7

6

6

4

3

2

1

1

1

1

1

1

1
Human, Maus
Wirt
189

130

9

6

4

4

2
Kaninchen
Passende Sekundärantikörper
Klonalität
197

146
Polyklonal
Konjugat
200

19

13

13

11

7

7

7

7

7

7

7

4

4

4

4

4

3

2

2

2

2

2

2

2

1

1
Dieser Vimentin Antikörper ist unkonjugiert
Applikation
282

132

99

99

96

64

49

40

39

28

23

20

8

5

4

4

2

2

2

1

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Homologie

B, Ha, Pr, Rat, Sh

Aufreinigung

This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen

This Vimentin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-90 amino acids from human Vimentin.

Klon

RB15215

Isotyp

Ig Fraction

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VIM Reaktivität: Human WB, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

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VIM Reaktivität: Human WB, IHC, ICC, IP Wirt: Maus Monoclonal C8 unconjugated

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VIM Reaktivität: Human, Schwein, Rind (Kuh), Huhn, Hund, Cat, Ziege WB, IHC, FACS, StM Wirt: Maus Monoclonal VM452 unconjugated

anti-Vimentin (VIM) (AA 2-466) antibody
VIM Reaktivität: Human, Maus, Ratte WB, IHC, ELISA, FACS Wirt: Maus Monoclonal 3A1F2 unconjugated

Applikationshinweise

IF: 1:25. WB: 1:1000. WB: 1:1000. WB: 1:1000. IHC-P: 1:10~50

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit

6 months
Target

Vimentin (VIM)

Andere Bezeichnung

Vimentin (VIM Produkte)

Synonyme

CTRCT30 antikoerper, cb28 antikoerper, vime antikoerper, vim antikoerper, vim1 antikoerper, vim2 antikoerper, VIM antikoerper, Vimentin antikoerper, vim4 antikoerper, vimentin antikoerper, vimentin L homeolog antikoerper, vimentin S homeolog antikoerper, VIM antikoerper, Vim antikoerper, vim antikoerper, vim.L antikoerper, vim.S antikoerper

Hintergrund

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.

Molekulargewicht

53652

Gen-ID

7431

NCBI Accession

NP_003371

UniProt

P08670

Pathways

Caspase Kaskade in der Apoptose