USP2 Antikörper (N-Term)

Produktnummer ABIN388877

Der Kaninchen Polyklonal Anti-USP2-Antikörper wurde für WB und IHC (p) validiert. Er ist geeignet, USP2 in Proben von Human und Ratte zu detektieren. Es sind 4+ Publikationen verfügbar.

Kurzübersicht für USP2 Antikörper (N-Term) (ABIN388877)

Target: USP2 (Ubiquitin Specific Peptidase 2 (USP2))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser USP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB4202

Produktdetails anti-USP2 Antikörper

Bindungsspezifität: AA 71-101, N-Term

Aufreinigung: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This USP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-101 amino acids from the N-terminal region of human USP2.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:1000. WB: 1:1000. WB: 1:1000. IHC-P: 1:10~50

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Referenzen für anti-USP2 Antikörper (ABIN388877)

Kitamura, Ishino, Shimamoto, Okabe, Miyamoto, Takahashi, Miyoshi: "Ubiquitin-Specific Protease 2 Modulates the Lipopolysaccharide-Elicited Expression of Proinflammatory Cytokines in Macrophage-like HL-60 Cells." in: Mediators of inflammation, Vol. 2017, pp. 6909415, (2018) (PubMed).

Kitamura, Kimura, Shimamoto, Okabe, Ito, Miyamoto, Naoe, Kikuguchi, Meek, Toda, Okamoto, Kanehira, Hase, Watarai, Ishizuka, El-Osta, Ohara, Miyoshi: "Ubiquitin-specific protease 2-69 in macrophages potentially modulates metainflammation." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 27, Issue 12, pp. 4940-53, (2013) (PubMed).

Stevenson, Sparks, Allende-Vega, Xirodimas, Lane, Saville: "The deubiquitinating enzyme USP2a regulates the p53 pathway by targeting Mdm2." in: The EMBO journal, Vol. 26, Issue 4, pp. 976-86, (2007) (PubMed).

Priolo, Tang, Brahamandan, Benassi, Sicinska, Ogino, Farsetti, Porrello, Finn, Zimmermann, Febbo, Loda: "The isopeptidase USP2a protects human prostate cancer from apoptosis." in: Cancer research, Vol. 66, Issue 17, pp. 8625-32, (2006) (PubMed).

Details zu USP2

Target: USP2 (Ubiquitin Specific Peptidase 2 (USP2))

Andere Bezeichnung: USP2

Hintergrund: Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Molekulargewicht: 68072

Gen-ID: 9099

NCBI Accession: NP_001230688, NP_004196, NP_741994

UniProt: O75604