CD73 Antikörper (C-Term)

Produktnummer ABIN388750

Produktdetails anti-CD73 Antikörper

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Bindungsspezifität: AA 520-550, C-Term

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CD73 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aufreinigung: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This CD73 (NT5E) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 520-550 amino acids from the C-terminal region of human CD73 (NT5E).

Klon: RB02846

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:1000. WB: 1:1000. WB: 1:500. WB: 1:1000. IHC-P: 1:10~50

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Haltbarkeit: 6 months

Referenzen für anti-CD73 Antikörper (ABIN388750)

Soleimanifar, Mortazavi, Nadri, Soleimani: "Conjunctiva derived mesenchymal stem cell (CJMSCs) as a potential platform for differentiation into corneal epithelial cells on bioengineered electrospun scaffolds." in: Journal of biomedical materials research. Part A, Vol. 105, Issue 10, pp. 2703-2711, (2018) (PubMed).

Yu, Wang, Lu, Wang, Li, Liao, Zhu, Lv, Zhi, Yu, Jin, Zou, Ou, Liu, Zhou: "Extracellular 5'-nucleotidase (CD73) promotes human breast cancer cells growth through AKT/GSK-3β/β-catenin/cyclinD1 signaling pathway." in: International journal of cancer, Vol. 142, Issue 5, pp. 959-967, (2018) (PubMed).

Tak, Jung, Kim, Park, Jun, Lee, Jung, Kirchner, Hwang, Song, Lee: "Protective role of hypoxia-inducible factor-1α-dependent CD39 and CD73 in fulminant acute liver failure." in: Toxicology and applied pharmacology, Vol. 314, pp. 72-81, (2017) (PubMed).

Cartón-García, Overeem, Nieto, Bazzocco, Dopeso, Macaya, Bilic, Landolfi, Hernandez-Losa, Schwartz, Ramon Y Cajal, van Ijzendoorn, Arango: "Myo5b knockout mice as a model of microvillus inclusion disease." in: Scientific reports, Vol. 5, pp. 12312, (2015) (PubMed).

Zhi, Wang, Yu, Yu, Zhang, Yin, Zhou: "Potential prognostic biomarker CD73 regulates epidermal growth factor receptor expression in human breast cancer." in: IUBMB life, Vol. 64, Issue 11, pp. 911-20, (2012) (PubMed).

Hart, Grenz, Gorzolla, Schittenhelm, Dalton, Eltzschig: "Hypoxia-inducible factor-1?-dependent protection from intestinal ischemia/reperfusion injury involves ecto-5'-nucleotidase (CD73) and the A2B adenosine receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 7, pp. 4367-74, (2011) (PubMed).

Zhi, Wang, Zhou, Yu, Jian, Tang, Yin, Zhou: "RNAi-mediated CD73 suppression induces apoptosis and cell-cycle arrest in human breast cancer cells." in: Cancer science, Vol. 101, Issue 12, pp. 2561-9, (2010) (PubMed).

Leth-Larsen, Lund, Hansen, Laenkholm, Tarin, Jensen, Ditzel: "Metastasis-related plasma membrane proteins of human breast cancer cells identified by comparative quantitative mass spectrometry." in: Molecular & cellular proteomics : MCP, Vol. 8, Issue 6, pp. 1436-49, (2009) (PubMed).

Hart, Henn, Köhler, Kloor, Mittelbronn, Gorzolla, Stahl, Eltzschig: "Role of extracellular nucleotide phosphohydrolysis in intestinal ischemia-reperfusion injury." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 22, Issue 8, pp. 2784-97, (2008) (PubMed).

Macdonald, Pike: "A simplified method for the preparation of detergent-free lipid rafts." in: Journal of lipid research, Vol. 46, Issue 5, pp. 1061-7, (2005) (PubMed).

Details zu CD73

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Andere Bezeichnung: CD73 (NT5E) (NT5E Produkte)

Hintergrund: Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70 kD subunits bound externally to the plasma membrane by a glycosyl phosphatidyl inositol linkage. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5E occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. It is not known whether the different enzymes are coded by different genes or result from different posttranslational modifications of a single coding sequence.

Molekulargewicht: 63368

Gen-ID: 4907

NCBI Accession: NP_001191742, NP_002517

UniProt: P21589

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process