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CD73 Antikörper (C-Term)

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch CD73 in WB und IHC (p). Er zeigt eine Reaktivität gegenüber Human und Maus und wurde in 10+ Publikationen erwähnt.
Produktnummer ABIN388750

Kurzübersicht für CD73 Antikörper (C-Term) (ABIN388750)

Target

Alle CD73 (NT5E) Antikörper anzeigen
CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reaktivität

  • 185
  • 77
  • 37
  • 19
  • 18
  • 16
  • 4
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Maus

Wirt

  • 107
  • 99
  • 22
  • 2
  • 2
  • 1
Kaninchen

Klonalität

  • 138
  • 94
  • 1
Polyklonal

Konjugat

  • 100
  • 21
  • 17
  • 9
  • 8
  • 5
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser CD73 Antikörper ist unkonjugiert

Applikation

  • 152
  • 125
  • 119
  • 59
  • 46
  • 40
  • 38
  • 27
  • 15
  • 14
  • 14
  • 7
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon

RB02846
  • Bindungsspezifität

    • 16
    • 13
    • 9
    • 9
    • 8
    • 5
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 520-550, C-Term

    Aufreinigung

    This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

    Immunogen

    This CD73 (NT5E) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 520-550 amino acids from the C-terminal region of human CD73 (NT5E).

    Isotyp

    Ig Fraction
  • Applikationshinweise

    WB: 1:1000. WB: 1:1000. WB: 1:500. WB: 1:1000. IHC-P: 1:10~50

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Buffer

    Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid freeze-thaw cycles.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

    Haltbarkeit

    6 months
  • Soleimanifar, Mortazavi, Nadri, Soleimani: "Conjunctiva derived mesenchymal stem cell (CJMSCs) as a potential platform for differentiation into corneal epithelial cells on bioengineered electrospun scaffolds." in: Journal of biomedical materials research. Part A, Vol. 105, Issue 10, pp. 2703-2711, (2018) (PubMed).

    Yu, Wang, Lu, Wang, Li, Liao, Zhu, Lv, Zhi, Yu, Jin, Zou, Ou, Liu, Zhou: "Extracellular 5'-nucleotidase (CD73) promotes human breast cancer cells growth through AKT/GSK-3β/β-catenin/cyclinD1 signaling pathway." in: International journal of cancer, Vol. 142, Issue 5, pp. 959-967, (2018) (PubMed).

    Tak, Jung, Kim, Park, Jun, Lee, Jung, Kirchner, Hwang, Song, Lee: "Protective role of hypoxia-inducible factor-1α-dependent CD39 and CD73 in fulminant acute liver failure." in: Toxicology and applied pharmacology, Vol. 314, pp. 72-81, (2017) (PubMed).

    Cartón-García, Overeem, Nieto, Bazzocco, Dopeso, Macaya, Bilic, Landolfi, Hernandez-Losa, Schwartz, Ramon Y Cajal, van Ijzendoorn, Arango: "Myo5b knockout mice as a model of microvillus inclusion disease." in: Scientific reports, Vol. 5, pp. 12312, (2015) (PubMed).

    Zhi, Wang, Yu, Yu, Zhang, Yin, Zhou: "Potential prognostic biomarker CD73 regulates epidermal growth factor receptor expression in human breast cancer." in: IUBMB life, Vol. 64, Issue 11, pp. 911-20, (2012) (PubMed).

    Hart, Grenz, Gorzolla, Schittenhelm, Dalton, Eltzschig: "Hypoxia-inducible factor-1?-dependent protection from intestinal ischemia/reperfusion injury involves ecto-5'-nucleotidase (CD73) and the A2B adenosine receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 7, pp. 4367-74, (2011) (PubMed).

    Zhi, Wang, Zhou, Yu, Jian, Tang, Yin, Zhou: "RNAi-mediated CD73 suppression induces apoptosis and cell-cycle arrest in human breast cancer cells." in: Cancer science, Vol. 101, Issue 12, pp. 2561-9, (2010) (PubMed).

    Leth-Larsen, Lund, Hansen, Laenkholm, Tarin, Jensen, Ditzel: "Metastasis-related plasma membrane proteins of human breast cancer cells identified by comparative quantitative mass spectrometry." in: Molecular & cellular proteomics : MCP, Vol. 8, Issue 6, pp. 1436-49, (2009) (PubMed).

    Hart, Henn, Köhler, Kloor, Mittelbronn, Gorzolla, Stahl, Eltzschig: "Role of extracellular nucleotide phosphohydrolysis in intestinal ischemia-reperfusion injury." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 22, Issue 8, pp. 2784-97, (2008) (PubMed).

    Macdonald, Pike: "A simplified method for the preparation of detergent-free lipid rafts." in: Journal of lipid research, Vol. 46, Issue 5, pp. 1061-7, (2005) (PubMed).

  • Target

    CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

    Andere Bezeichnung

    CD73 (NT5E)

    Hintergrund

    Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70 kD subunits bound externally to the plasma membrane by a glycosyl phosphatidyl inositol linkage. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5E occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. It is not known whether the different enzymes are coded by different genes or result from different posttranslational modifications of a single coding sequence.

    Molekulargewicht

    63368

    Gen-ID

    4907

    NCBI Accession

    NP_001191742, NP_002517

    UniProt

    P21589

    Pathways

    Synaptic Membrane, Ribonucleoside Biosynthetic Process
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