Beclin 1 Antikörper (AA 210-239)

Produktnummer ABIN388546

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Beclin 1 in WB, FACS und IHC (p). Er zeigt eine Reaktivität gegenüber Human und Maus und wurde in 6+ Publikationen erwähnt.

Kurzübersicht für Beclin 1 Antikörper (AA 210-239) (ABIN388546)

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Beclin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB7463

Produktdetails anti-Beclin 1 Antikörper

Bindungsspezifität: AA 210-239

Homologie: B, Pig, Rat

Aufreinigung: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This Beclin 1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 210-239 amino acids from human Beclin 1.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:4000. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Referenzen für anti-Beclin 1 Antikörper (ABIN388546)

Pla, Pascual, Guerri: "Autophagy Constitutes a Protective Mechanism against Ethanol Toxicity in Mouse Astrocytes and Neurons." in: PLoS ONE, Vol. 11, Issue 4, pp. e0153097, (2016) (PubMed).

Li, Xiong, Shi, Xiao, Qi, Meng: "IKKβ/NFκBp65 activated by interleukin-13 targets the autophagy-related genes LC3B and beclin 1 in fibroblasts co-cultured with breast cancer cells." in: Experimental and therapeutic medicine, Vol. 11, Issue 4, pp. 1259-1264, (2016) (PubMed).

Jia, Jin, Xiao, Dong, Wang, Fan, Xu, Meng, Li, Lv: "Lipoxin A4 methyl ester alleviates vascular cognition impairment by regulating the expression of proteins related to autophagy and ER stress in the rat hippocampus." in: Cellular & molecular biology letters, Vol. 20, Issue 3, pp. 475-87, (2015) (PubMed).

Liu, He, von Rütte, Yousefi, Hunger, Simon: "Down-regulation of autophagy-related protein 5 (ATG5) contributes to the pathogenesis of early-stage cutaneous melanoma." in: Science translational medicine, Vol. 5, Issue 202, pp. 202ra123, (2013) (PubMed).

Dhule, Penfornis, Frazier, Walker, Feldman, Tan, He, Alb, John, Pochampally: "Curcumin-loaded ?-cyclodextrin liposomal nanoparticles as delivery vehicles for osteosarcoma." in: Nanomedicine : nanotechnology, biology, and medicine, Vol. 8, Issue 4, pp. 440-51, (2012) (PubMed).

Wang, Whiteman, Lian, Wang, Singh, Huang, Denmark: "A non-canonical MEK/ERK signaling pathway regulates autophagy via regulating Beclin 1." in: The Journal of biological chemistry, Vol. 284, Issue 32, pp. 21412-24, (2009) (PubMed).

Details zu Beclin 1

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Andere Bezeichnung: Beclin 1

Hintergrund: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). Beclin 1 plays a role in two fundamentally important cell biological pathways: autophagy and apoptosis. Beclin 1 is thought to function as a VPS and autophagy protein as part of a complex with Class III PI3 kinase, Vps34.

Molekulargewicht: 51896

Gen-ID: 8678

NCBI Accession: NP_003757

UniProt: Q14457

Pathways: Autophagie