ATG4B Antikörper (N-Term)

Produktnummer ABIN388494

Dieses Anti-ATG4B-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von ATG4B in WB und IHC (p). Geeignet für Human und Maus. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.

Kurzübersicht für ATG4B Antikörper (N-Term) (ABIN388494)

Target: ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATG4B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB7496

Produktdetails anti-ATG4B Antikörper

Bindungsspezifität: AA 16-45, N-Term

Aufreinigung: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This ATG4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-45 amino acids from the N-terminal region of human ATG4B.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:1000. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Referenzen für anti-ATG4B Antikörper (ABIN388494)

Yang, Zhao, Mei, Jiang, Geng, Li, Yao, Liu, Kong, Cao: "HMGA2 plays an important role in Cr (VI)-induced autophagy." in: International journal of cancer, Vol. 141, Issue 5, pp. 986-997, (2017) (PubMed).

Garneau, Alvarez, Paquin, Lussier, Rancourt, Tremblay, Beaulieu, Rivard: "Nuclear expression of E2F4 induces cell death via multiple pathways in normal human intestinal epithelial crypt cells but not in colon cancer cells." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 293, Issue 4, pp. G758-72, (2007) (PubMed).

Details zu ATG4B

Target: ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))

Andere Bezeichnung: ATG4B

Hintergrund: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4 is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.

Molekulargewicht: 44294

Gen-ID: 23192

NCBI Accession: NP_037457, NP_847896

UniProt: Q9Y4P1

Pathways: Autophagie