PUMA Antikörper (AA 119-154)

Produktnummer ABIN388112

Der Kaninchen Polyklonal Anti-PUMA-Antikörper wurde für WB, IF, FACS und IHC (p) validiert. Er ist geeignet, PUMA in Proben von Human zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für PUMA Antikörper (AA 119-154) (ABIN388112)

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PUMA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB01353

Produktdetails anti-PUMA Antikörper

Bindungsspezifität: AA 119-154

Homologie: M, Rat

Aufreinigung: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This Puma BH3 Domain antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-154 amino acids from human Puma BH3 Domain.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: IF: 1:10~50. WB: 1:1000. IHC-P: 1:10~50. FC: 1:10~50

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Referenzen für anti-PUMA Antikörper (ABIN388112)

Vesela, Matalova: "Detection of Bim and Puma in mouse hair follicles using immunofluorescence and TUNEL assay double staining." in: Biotechnic & histochemistry : official publication of the Biological Stain Commission, pp. 1-7, (2015) (PubMed).

Details zu PUMA

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Andere Bezeichnung: Puma

Hintergrund: PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

Molekulargewicht: 20532

Gen-ID: 27113

NCBI Accession: NP_001120712, NP_001120713, NP_001120714, NP_055232

UniProt: Q9BXH1

Pathways: p53 Signalweg, Positive Regulation of Endopeptidase Activity