Ubiquitin Antikörper (N-Term)

Produktnummer ABIN388039

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Ubiquitin in WB und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte und wurde in 1 Publikation erwähnt.

Kurzübersicht für Ubiquitin Antikörper (N-Term) (ABIN388039)

Target: Ubiquitin (Ub)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Ubiquitin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB0643

Produktdetails anti-Ubiquitin Antikörper

Bindungsspezifität: AA 1-32, N-Term

Homologie: B, D, Pr, Pig, Sh, C, Hs, Ha, X, Rb

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This Ubiquitin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-32 amino acids from the N-terminal region of human Ubiquitin.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:2000. IHC-P-Leica: 1:500. IHC-P-Leica: 1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Referenzen für anti-Ubiquitin Antikörper (ABIN388039)

Ouyang, Ali, Ravichandran, Dong, Qiu, MacKenzie, Dhe-Paganon, Arrowsmith, Zhai: "Protein aggregates are recruited to aggresome by histone deacetylase 6 via unanchored ubiquitin C termini." in: The Journal of biological chemistry, Vol. 287, Issue 4, pp. 2317-27, (2012) (PubMed).

Details zu Ubiquitin

Target: Ubiquitin (Ub)

Andere Bezeichnung: Ubiquitin

Hintergrund: This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.

Molekulargewicht: 77039

Gen-ID: 7314

UniProt: P0CG48

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway