His Tag Antikörper

Produktnummer ABIN387901

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch His Tag in WB. Es wurde in 1 Publikation zitiert.

Kurzübersicht für His Tag Antikörper (ABIN387901)

Target: His Tag

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser His Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: RB19955

Produktdetails anti-His Tag Antikörper

Sequenz: HHHHHH

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: Poly-HIS peptide were used to produced this antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:2000. WB: 1:2000. WB: 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Referenzen für anti-His Tag Antikörper (ABIN387901)

Anderson, Robson, Jiang, Malmirchegini, Fierobe, Lazazzera, Clubb: "Assembly of minicellulosomes on the surface of Bacillus subtilis." in: Applied and environmental microbiology, Vol. 77, Issue 14, pp. 4849-58, (2011) (PubMed).

Details zu His Tag

Target: His Tag

Andere Bezeichnung: 6 X His Tag

Substanzklasse: Tag

Hintergrund: Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18 provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. Abgent's anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.