His Tag Antikörper (FITC)

Produktnummer ABIN387722

Der Maus Monoklonal Anti-His Tag-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, His Tag in Proben von zu detektieren. Es sind 5+ Publikationen verfügbar.

Kurzübersicht für His Tag Antikörper (FITC) (ABIN387722)

Target: His Tag

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser His Tag Antikörper ist konjugiert mit FITC

Applikation: Western Blotting (WB), ELISA

Klon: 6G2A9

Produktdetails anti-His Tag Antikörper (FITC)

Marke: THE™

Sequenz: HHHHHH

Spezifität: THE^TM His Tag Antibody [FITC], mAb, Mouserecognizes C-terminal, N-terminal, and internal His tagged fusion proteins.

Produktmerkmale: This product is suitable for detecting the expression level of His fusion proteins by various immunoassays.Absorption Wavelength: 492 nmEmission Wavelength: 518 nmFluorochrome/Protein Ratio: >3 moles FITC per mole antibody

Aufreinigung: Protein A affinity column

Immunogen: A synthetic peptide HHHHHH coupled - KLH

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescence (IF): 0.1-2 µg/mL
Flow cytometry: 0.1-2 µg for 1 x 10^6 cells
Other applications: user-optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 1 % BSA and 0.01 % Sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freezing and thawing cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Referenzen für anti-His Tag Antikörper (FITC) (ABIN387722)

Yang, Wahlmüller, Sarg, Furtmüller, Geiger: "A+-helix of protein C inhibitor (PCI) is a cell-penetrating peptide that mediates cell membrane permeation of PCI." in: The Journal of biological chemistry, Vol. 290, Issue 5, pp. 3081-91, (2015) (PubMed).

Jarmander, Janoschek, Lundh, Larsson, Gustavsson: "Process optimization for increased yield of surface-expressed protein in Escherichia coli." in: Bioprocess and biosystems engineering, Vol. 37, Issue 8, pp. 1685-93, (2014) (PubMed).

An, Budd, Kanost, Michel: "Characterization of a regulatory unit that controls melanization and affects longevity of mosquitoes." in: Cellular and molecular life sciences : CMLS, Vol. 68, Issue 11, pp. 1929-39, (2011) (PubMed).

Oliveira, Lindblad: "Novel insights into the regulation of LexA in the cyanobacterium Synechocystis sp. Strain PCC 6803." in: Journal of bacteriology, Vol. 193, Issue 15, pp. 3804-14, (2011) (PubMed).

Li, Wang, Zhou, Yang, Bhaskar, Hu, Sun, Miao: "Cloning and expression of a cellulase gene in the silkworm, Bombyx mori by improved Bac-to-Bac/BmNPV baculovirus expression system." in: Molecular biology reports, Vol. 37, Issue 8, pp. 3721-8, (2010) (PubMed).

Details zu His Tag

Target: His Tag

Substanzklasse: Tag