HA-Tag Antikörper

Produktnummer ABIN387711

Zitiert in 7+ Publikationen. Der Maus Monoklonal anti-HA-Tag Antikörper (Klon 5E11D8) (ABIN387711) detektiert spezifisch HA-Tag in WB und ELISA.

Kurzübersicht für HA-Tag Antikörper (ABIN387711)

Target: HA-Tag

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HA-Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Klon: 5E11D8

Produktdetails anti-HA-Tag Antikörper

Sequenz: YPYDVPDYA

Spezifität: THE^TM HA Tag Antibody, mAb, Mouse (A01244) recognizes C-terminal, N-terminal, and internal HA tagged-fusion proteins.

Produktmerkmale: Anti-HA-tag mAb (ABIN387711) is a highly sensitive monoclonal antibody applicable to HA-tagged fusion protein detection. It belongs to our elite antibody collection. HA, or human influenza hemagglutinin, is a surface glycoprotein required for human influenza virus infectivity. The HA tag does not appear to interfere with the bioactivity or the biodistribution of recombinant proteins. Anti-HA-tag mAb (Antibodies-Online, ABIN387711) is a high-affinity monoclonal antibody that can be used to detect HA-tagged proteins. This product detects HA tags derived from an HA molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors.

Aufreinigung: Protein A affinity column

Immunogen: A KLH coupled synthetic peptide (YPYDVPDYA)

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mL
Immunoprecipitation: 1 µg/mL
Other applications: user-optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freezing and thawing cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.

Referenzen für anti-HA-Tag Antikörper (ABIN387711)

Milewska, Kindler, Vkovski, Zeglen, Ochman, Thiel, Rajfur, Pyrc: "APOBEC3-mediated restriction of RNA virus replication." in: Scientific reports, Vol. 8, Issue 1, pp. 5960, (2019) (PubMed).

Naskalska, Dabrowska, Nowak, Szczepanski, Jasik, Milewska, Ochman, Zeglen, Rajfur, Pyrc: "Novel coronavirus-like particles targeting cells lining the respiratory tract." in: PLoS ONE, Vol. 13, Issue 9, pp. e0203489, (2019) (PubMed).

Cheng, Yu, Liu, Deng, Ma, Wang: "Functional analysis of bovine Nramp1 and production of transgenic cloned embryos in vitro." in: Zygote (Cambridge, England), Vol. 23, Issue 1, pp. 83-92, (2015) (PubMed).

Tanner, Liu, Oberste, Pallansch, Collett, Kirkegaard: "Dominant drug targets suppress the emergence of antiviral resistance." in: eLife, Vol. 3, (2014) (PubMed).

Pillay, Selland, Fleisch, Leighton, Cheng, Famulski, Ritzel, March, Wang, Allison, Waskiewicz: "Evaluating the mutagenic activity of targeted endonucleases containing a Sharkey FokI cleavage domain variant in zebrafish." in: Zebrafish, Vol. 10, Issue 3, pp. 353-64, (2013) (PubMed).

Shulga, Anderson, Topham, Epand: "Phosphatidylinositol-4-phosphate 5-kinase isoforms exhibit acyl chain selectivity for both substrate and lipid activator." in: The Journal of biological chemistry, Vol. 287, Issue 43, pp. 35953-63, (2012) (PubMed).

Borroto-Escuela, Romero-Fernandez, García-Negredo, Correia, Garriga, Fuxe, Ciruela: "Dissecting the conserved NPxxY motif of the M3 muscarinic acetylcholine receptor: critical role of Asp-7.49 for receptor signaling and multiprotein complex formation." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 28, Issue 5, pp. 1009-22, (2011) (PubMed).

Details zu HA-Tag

Target: HA-Tag

Andere Bezeichnung: HA Tag

Substanzklasse: Tag