beta Actin Antikörper (HRP)

Produktnummer ABIN7477941

Dieses Anti-beta Actin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von beta Actin in WB und ELISA. Geeignet für Human, Maus, Ratte, Huhn, Kaninchen, Schwein, Rind (Kuh), Hamster, Ziege und Fisch. Dieses Primary Antibody wurde in 3+ Publikationen zitiert.

Kurzübersicht für beta Actin Antikörper (HRP) (ABIN7477941)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reaktivität: Human, Maus, Ratte, Huhn, Kaninchen, Schwein, Rind (Kuh), Hamster, Ziege, Fisch

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser beta Actin Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA

Klon: 2D1D10

Produktdetails anti-beta Actin Antikörper (HRP)

Spezifität: Anti-beta-Actin [HRP] Monoclonal Antibody (Mouse) reacts with mouse, rabbit, chicken, human, hamster, cow, goat, fish, and pig substrates. It has not yet been tested in other species.

Kreuzreaktivität (Details): Cross reactivity in other species has not yet been tested.

Produktmerkmale: This product provides a useful and serves as a specific tool in the study of the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton. Do not add azide because it inhibits the activity of HRP.

Aufreinigung: Protein G affinity column

Immunogen: A synthetic peptide DDDIAALVVDNGSG coupled - KLH

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.1-1.0 µg/mL
Western blot: 0.1-1.0 µg/mL
Other applications: user-optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 1 % BSA and 0.01 % Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.

Referenzen für anti-beta Actin Antikörper (HRP) (ABIN7477941)

Ferreira, Wang: "Optimization of oncogene expression through intra-population competition." in: Biotechnology journal, Vol. 8, Issue 12, pp. 1476-84, (2013) (PubMed).

Jiménez-Soto, Clausen, Sprenger, Ertl, Haas: "Dynamics of the Cag-type IV secretion system of Helicobacter pylori as studied by bacterial co-infections." in: Cellular microbiology, Vol. 15, Issue 11, pp. 1924-37, (2013) (PubMed).

Berry, Shin, Lightfoot, Janknecht: "Oncogenic features of the JMJD2A histone demethylase in breast cancer." in: International journal of oncology, Vol. 41, Issue 5, pp. 1701-6, (2012) (PubMed).

Details zu beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Andere Bezeichnung: beta Actin

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction