His Tag Antikörper (HRP)

Produktnummer ABIN387701

Der Maus Monoklonal Anti-His Tag-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, His Tag in Proben von zu detektieren. Es sind 6+ Publikationen verfügbar.

Kurzübersicht für His Tag Antikörper (HRP) (ABIN387701)

Target: His Tag

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser His Tag Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA

Klon: 6G2A9

Produktdetails anti-His Tag Antikörper (HRP)

Marke: THE™

Sequenz: HHHHHH

Spezifität: THE^TM His Tag Antibody [HRP], mAb, Mouse recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.

Produktmerkmale: This product is suitable for detecting the expression level of His fusion proteins or His by various immunoassays.

Aufreinigung: Protein A affinity column

Immunogen: A synthetic peptide HHHHHH coupled - KLH

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.5 µg/mL
Western blot: 0.1-1.0 µg/mL
Other applications: user-optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 1 % BSA and 0.01 % Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Referenzen für anti-His Tag Antikörper (HRP) (ABIN387701)

Samelson, Gore, Whiting, Nygren, Purkey, Colledge, Langeberg, DellAcqua, Zweifel, Scott: "A-kinase Anchoring Protein 79/150 Recruits Protein Kinase C to Phosphorylate Roundabout Receptors." in: The Journal of biological chemistry, Vol. 290, Issue 22, pp. 14107-19, (2015) (PubMed).

Almeida, Abreu, Matos, Fraga, Fernandes, Macedo, Gutiérrez-Gallego, Pereira, Carvalho, Macedo-Ribeiro: "SUMOylation of the brain-predominant Ataxin-3 isoform modulates its interaction with p97." in: Biochimica et biophysica acta, Vol. 1852, Issue 9, pp. 1950-9, (2015) (PubMed).

Isik, Sliepen, van Montfort, Sanders: "Enhanced immunogenicity of HIV-1 envelope gp140 proteins fused to APRIL." in: PLoS ONE, Vol. 9, Issue 9, pp. e107683, (2014) (PubMed).

Vire, Skarzynski, Thomas, Nelson, David, Aue, Burke, Rader, Wiestner: "Harnessing the fc? receptor for potent and selective cytotoxic therapy of chronic lymphocytic leukemia." in: Cancer research, Vol. 74, Issue 24, pp. 7510-20, (2014) (PubMed).

Melnikow, Xu, Liu, Bell, Ghedin, Unnasch, Lustigman: "A potential role for the interaction of Wolbachia surface proteins with the Brugia malayi glycolytic enzymes and cytoskeleton in maintenance of endosymbiosis." in: PLoS neglected tropical diseases, Vol. 7, Issue 4, pp. e2151, (2013) (PubMed).

Jarmander, Gustavsson, Do, Samuelson, Larsson: "A dual tag system for facilitated detection of surface expressed proteins in Escherichia coli." in: Microbial cell factories, Vol. 11, pp. 118, (2012) (PubMed).

Details zu His Tag

Target: His Tag

Substanzklasse: Tag