Phosphotyrosine Antikörper (PE)

Produktnummer ABIN376479

Produktdetails anti-Phosphotyrosine Antikörper (PE)

Target: Phosphotyrosine

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Phosphotyrosine Antikörper ist konjugiert mit PE

Applikation: ELISA

Spezifität: Tyrosine-phosphorylated proteins

Produktmerkmale: Mouse Anti-Phosphotyrosine-PE

Aufreinigung: Purified

Immunogen: Phosphotyrosine conjugated to carrier proteins

Klon: PY20

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

• Applications: ELISA - Quality tested , FLISA - Quality tested WB - Reported in literature , IP - Reported in literature , IHC-PS - Reported in literature , IHC-WM - Reported in literature , ICC - Reported in literature , FC - Reported in literature , Microarray - Reported in literature , Purification - Reported in literature
• Working Dilutions: ELISA AP conjugate 1:1,000 - 1:2,000 HRP conjugate 1:1,000 - 1:4,000 BIOT conjugate 1:5,000 - 1:10,000 FLISA FITC conjugate 1:200 - 1:400 PE conjugate ≤ 1 g/mL

Probenmenge: 1 mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C

Details zu Phosphotyrosine

Target: Phosphotyrosine

Abstract: Phosphotyrosine Produkte

Substanzklasse: Amino Acid

Hintergrund: Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g. via growth factors) during normal growth and development, and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation are cell surface glycoproteins. Antibody to phosphotyrosine enables the detection, isolation and characterization of proteins containing phosphotyrosine. PY20 also prevents internalization of activated receptors, e.g. EGFr, when microinjected into cells. The affinity of PY20 for phosphotyrosine is approximately 10 -6 to 10 -7 M. PY20 binding to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by phosphoserine, phosphothreonine or free phosphate.