FTH1 Antikörper (C-Term, Heavy Chain)

Produktnummer ABIN375067

Dieser Ziege Polyklonal Antikörper detektiert spezifisch FTH1 in WB, IHC (p) und EIA. Es zeigt Reaktivität gegenüber Proben von Human und Hund.Es wurde in 1 Publikation zitiert.

Kurzübersicht für FTH1 Antikörper (C-Term, Heavy Chain) (ABIN375067)

Target: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Reaktivität: Human, Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser FTH1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-FTH1 Antikörper

Bindungsspezifität: C-Term, Heavy Chain

Sequenz: C-DKHTLGDSDN ES

Spezifität: This antibody recognizes FTH1 at C-term.

Kreuzreaktivität (Details): Species reactivity (expected):Canine.
Species reactivity (tested):Human.

Aufreinigung: Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the C-Terminus of the protein sequence according to NP_002023.2. Genename: FTH1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Detection Limit: 1/8000. Western blot: 0.03-0.1 μg/mL. Approx.22 kDa band observed in Human Brain (Cerebellum),Liver and Placenta lysates. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-FTH1 Antikörper (ABIN375067)

Sammarco, Ditch, Banerjee, Grabczyk: "Ferritin L and H subunits are differentially regulated on a post-transcriptional level." in: The Journal of biological chemistry, Vol. 283, Issue 8, pp. 4578-87, (2008) (PubMed).

Details zu FTH1

Target: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Andere Bezeichnung: Ferritin Heavy Chain (FTH1)

Hintergrund: Ferritin is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis by sequestering and storing iron in a non-toxic and soluble form. It forms a holoenzyme of ~450 kDa, consisting of 24 subunits of two types, H (heavy, 21 kDa) and L (light, 19 kDa), and is capable of storing up to 4,500 atoms of ferric iron. Depending on the tissue type and physiological status of the cell, the ratio of H to L subunits in ferritin can vary widely. Ferritin is found in the liver, spleen, kidney and heart, with smaller amounts being found in blood. Serum ferritin levels serve as an indicator of the amount of iron stored in the body. Serum ferritin is the most sensitive test for anaemia, and is also used as a marker for restless leg syndrome, hemochromatosis and porphyria. As ferritin is an acute-phase reactant, it is often elevated during infection. Defects in ferritin proteins are associated with several neurodegenerative diseases.Synonyms: Cell proliferation-inducing gene 15 protein, EC=1.16.3.1, FTH, FTH-1, FTHL6, Ferritin H subunit

Molekulargewicht: 21.2kDa (NP_002023.2)

Gen-ID: 2495

NCBI Accession: NP_002023

UniProt: P02794

Pathways: Transition Metal Ion Homeostasis